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Determination of Sulpiride by Capillary Electrophoresis with End-Column Electrogenerated Chemiluminescence Detection
Capillary electrophoresis (CE) with tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)3(2+)]-electrogenerated chemiluminescence (ECL) detection is a promising method for clinical analysis. In this study, a method combining CE with Ru(bpy)3(2+) ECL (CE-ECL) detection that can be applied to amine-contain...
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| Published in: | Clinical chemistry (Baltimore, Md.) Md.), 2002-07, Vol.48 (7), p.1049-1058 |
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| container_title | Clinical chemistry (Baltimore, Md.) |
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| creator | Liu, Jifeng Cao, Weidong Qiu, Haibo Sun, Xiuhua Yang, Xiurong Wang, Erkang |
| description | Capillary electrophoresis (CE) with tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)3(2+)]-electrogenerated chemiluminescence (ECL) detection is a promising method for clinical analysis. In this study, a method combining CE with Ru(bpy)3(2+) ECL (CE-ECL) detection that can be applied to amine-containing clinical species was developed, and the performance of CE-ECL as a quantitative method for determination of sulpiride in human plasma or urine was evaluated.
Sulpiride was separated by capillary zone electrophoresis in uncoated fused-silica capillaries [50 cm x 25 microm (i.d.)] filled with phosphate buffer (pH 8.0) and a driving voltage of +15 kV, with end-column Ru(bpy)3(2+) ECL detection. A platinum disc electrode was used as working electrode. Sulpiride in human plasma or urine samples (100 microL) was extracted by a double-step liquid-liquid extraction procedure, dried under nitrogen at 35 degrees C in a water bath, and reconstituted with 100 microL of filtered water. The extraction solvent was ethyl acetate-dichloromethane (5:1 by volume).
Under optimum conditions (pH 8.0 phosphate buffer, injection for 6 s at 10 kV, and +1.2 V as detection potential), separation of sulpiride was accomplished within 4 min. The calibration curve was linear over a concentration range of 0.05-25.0 micromol/L, and the limit of detection was 2.9 x 10(-8) mol/L for sulpiride. Intra- and interday CVs for ECL intensities were |
| doi_str_mv | 10.1093/clinchem/48.7.1049 |
| format | article |
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Sulpiride was separated by capillary zone electrophoresis in uncoated fused-silica capillaries [50 cm x 25 microm (i.d.)] filled with phosphate buffer (pH 8.0) and a driving voltage of +15 kV, with end-column Ru(bpy)3(2+) ECL detection. A platinum disc electrode was used as working electrode. Sulpiride in human plasma or urine samples (100 microL) was extracted by a double-step liquid-liquid extraction procedure, dried under nitrogen at 35 degrees C in a water bath, and reconstituted with 100 microL of filtered water. The extraction solvent was ethyl acetate-dichloromethane (5:1 by volume).
Under optimum conditions (pH 8.0 phosphate buffer, injection for 6 s at 10 kV, and +1.2 V as detection potential), separation of sulpiride was accomplished within 4 min. The calibration curve was linear over a concentration range of 0.05-25.0 micromol/L, and the limit of detection was 2.9 x 10(-8) mol/L for sulpiride. Intra- and interday CVs for ECL intensities were <6%. Extraction recoveries of sulpiride were 95.6-101% with CVs of 2.9-6.0%. The method was clinically validated for patient plasma and urine samples.
CE combined with Ru(bpy)3(2+) ECL is reproducible, precise, selective, and enables the analysis of sulpiride in human plasma and urine. It thus is of value for rapid and efficient analysis of amine-containing analytes of clinical interest.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1093/clinchem/48.7.1049</identifier><identifier>PMID: 12089174</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>2,2'-Dipyridyl - analogs & derivatives ; 2,2'-Dipyridyl - chemistry ; Acetates ; Adult ; Antidepressive Agents, Second-Generation - analysis ; Antidepressive Agents, Second-Generation - blood ; Antidepressive Agents, Second-Generation - urine ; Antipsychotic Agents - analysis ; Antipsychotic Agents - blood ; Antipsychotic Agents - urine ; Biological and medical sciences ; Dopamine Antagonists - analysis ; Dopamine Antagonists - blood ; Dopamine Antagonists - urine ; Electrochemistry ; Electrophoresis, Capillary ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Luminescent Measurements ; Medical sciences ; Methylene Chloride ; Middle Aged ; Miscellaneous. Technology ; Organometallic Compounds - chemistry ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Receptors, Dopamine D2 - drug effects ; Ruthenium ; Solvents ; Sulpiride - analysis ; Sulpiride - blood ; Sulpiride - urine</subject><ispartof>Clinical chemistry (Baltimore, Md.), 2002-07, Vol.48 (7), p.1049-1058</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-248a1c7ecd2ee8c0e1a2f9ae717260cb0fc4307026789b363eb622287a7162633</citedby><cites>FETCH-LOGICAL-c404t-248a1c7ecd2ee8c0e1a2f9ae717260cb0fc4307026789b363eb622287a7162633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13738867$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12089174$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Jifeng</creatorcontrib><creatorcontrib>Cao, Weidong</creatorcontrib><creatorcontrib>Qiu, Haibo</creatorcontrib><creatorcontrib>Sun, Xiuhua</creatorcontrib><creatorcontrib>Yang, Xiurong</creatorcontrib><creatorcontrib>Wang, Erkang</creatorcontrib><title>Determination of Sulpiride by Capillary Electrophoresis with End-Column Electrogenerated Chemiluminescence Detection</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>Capillary electrophoresis (CE) with tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)3(2+)]-electrogenerated chemiluminescence (ECL) detection is a promising method for clinical analysis. In this study, a method combining CE with Ru(bpy)3(2+) ECL (CE-ECL) detection that can be applied to amine-containing clinical species was developed, and the performance of CE-ECL as a quantitative method for determination of sulpiride in human plasma or urine was evaluated.
Sulpiride was separated by capillary zone electrophoresis in uncoated fused-silica capillaries [50 cm x 25 microm (i.d.)] filled with phosphate buffer (pH 8.0) and a driving voltage of +15 kV, with end-column Ru(bpy)3(2+) ECL detection. A platinum disc electrode was used as working electrode. Sulpiride in human plasma or urine samples (100 microL) was extracted by a double-step liquid-liquid extraction procedure, dried under nitrogen at 35 degrees C in a water bath, and reconstituted with 100 microL of filtered water. The extraction solvent was ethyl acetate-dichloromethane (5:1 by volume).
Under optimum conditions (pH 8.0 phosphate buffer, injection for 6 s at 10 kV, and +1.2 V as detection potential), separation of sulpiride was accomplished within 4 min. The calibration curve was linear over a concentration range of 0.05-25.0 micromol/L, and the limit of detection was 2.9 x 10(-8) mol/L for sulpiride. Intra- and interday CVs for ECL intensities were <6%. Extraction recoveries of sulpiride were 95.6-101% with CVs of 2.9-6.0%. The method was clinically validated for patient plasma and urine samples.
CE combined with Ru(bpy)3(2+) ECL is reproducible, precise, selective, and enables the analysis of sulpiride in human plasma and urine. It thus is of value for rapid and efficient analysis of amine-containing analytes of clinical interest.</description><subject>2,2'-Dipyridyl - analogs & derivatives</subject><subject>2,2'-Dipyridyl - chemistry</subject><subject>Acetates</subject><subject>Adult</subject><subject>Antidepressive Agents, Second-Generation - analysis</subject><subject>Antidepressive Agents, Second-Generation - blood</subject><subject>Antidepressive Agents, Second-Generation - urine</subject><subject>Antipsychotic Agents - analysis</subject><subject>Antipsychotic Agents - blood</subject><subject>Antipsychotic Agents - urine</subject><subject>Biological and medical sciences</subject><subject>Dopamine Antagonists - analysis</subject><subject>Dopamine Antagonists - blood</subject><subject>Dopamine Antagonists - urine</subject><subject>Electrochemistry</subject><subject>Electrophoresis, Capillary</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Luminescent Measurements</subject><subject>Medical sciences</subject><subject>Methylene Chloride</subject><subject>Middle Aged</subject><subject>Miscellaneous. Technology</subject><subject>Organometallic Compounds - chemistry</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Receptors, Dopamine D2 - drug effects</subject><subject>Ruthenium</subject><subject>Solvents</subject><subject>Sulpiride - analysis</subject><subject>Sulpiride - blood</subject><subject>Sulpiride - urine</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNpFUE1v1DAQtSpQu5T-gR6QL3BL66-1nSMKC61UiQNwthxn0hg5TrATRf33eNWt9jSamTfvvXkI3VJyR0nN713w0Q0w3gt9p8pI1BdoR_ecVHov6Tu0I4TUVU2FukIfcv5bWqG0vERXlBFdUyV2aPkGC6TRR7v4KeKpx7_WMPvkO8DtC27s7EOw6QUfArglTfMwJcg-480vAz7ErmqmsI7xbf8MEZJdoMNNcebLykfIDqIDfJRyR5mP6H1vQ4abU71Gf74ffjcP1dPPH4_N16fKCSKWigltqVPgOgagHQFqWV9bUFQxSVxLeic4UYRJpeuWSw6tZIxpZRWVTHJ-jb688s5p-rdCXszoi5fyUIRpzUZRLfe6ZgXIXoEuTTkn6M2c_FjeNpSYY9bmLWsjtFHmmHU5-nRiX9sRuvPJKdwC-HwC2Oxs6JONzuczjiuutVRnm4N_HjafwOTRhlBoqdm27az4HyB0mOY</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Liu, Jifeng</creator><creator>Cao, Weidong</creator><creator>Qiu, Haibo</creator><creator>Sun, Xiuhua</creator><creator>Yang, Xiurong</creator><creator>Wang, Erkang</creator><general>Am Assoc Clin Chem</general><general>American Association for Clinical Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Determination of Sulpiride by Capillary Electrophoresis with End-Column Electrogenerated Chemiluminescence Detection</title><author>Liu, Jifeng ; Cao, Weidong ; Qiu, Haibo ; Sun, Xiuhua ; Yang, Xiurong ; Wang, Erkang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-248a1c7ecd2ee8c0e1a2f9ae717260cb0fc4307026789b363eb622287a7162633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>2,2'-Dipyridyl - analogs & derivatives</topic><topic>2,2'-Dipyridyl - chemistry</topic><topic>Acetates</topic><topic>Adult</topic><topic>Antidepressive Agents, Second-Generation - analysis</topic><topic>Antidepressive Agents, Second-Generation - blood</topic><topic>Antidepressive Agents, Second-Generation - urine</topic><topic>Antipsychotic Agents - analysis</topic><topic>Antipsychotic Agents - blood</topic><topic>Antipsychotic Agents - urine</topic><topic>Biological and medical sciences</topic><topic>Dopamine Antagonists - analysis</topic><topic>Dopamine Antagonists - blood</topic><topic>Dopamine Antagonists - urine</topic><topic>Electrochemistry</topic><topic>Electrophoresis, Capillary</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Luminescent Measurements</topic><topic>Medical sciences</topic><topic>Methylene Chloride</topic><topic>Middle Aged</topic><topic>Miscellaneous. Technology</topic><topic>Organometallic Compounds - chemistry</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Receptors, Dopamine D2 - drug effects</topic><topic>Ruthenium</topic><topic>Solvents</topic><topic>Sulpiride - analysis</topic><topic>Sulpiride - blood</topic><topic>Sulpiride - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Jifeng</creatorcontrib><creatorcontrib>Cao, Weidong</creatorcontrib><creatorcontrib>Qiu, Haibo</creatorcontrib><creatorcontrib>Sun, Xiuhua</creatorcontrib><creatorcontrib>Yang, Xiurong</creatorcontrib><creatorcontrib>Wang, Erkang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Jifeng</au><au>Cao, Weidong</au><au>Qiu, Haibo</au><au>Sun, Xiuhua</au><au>Yang, Xiurong</au><au>Wang, Erkang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Sulpiride by Capillary Electrophoresis with End-Column Electrogenerated Chemiluminescence Detection</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>48</volume><issue>7</issue><spage>1049</spage><epage>1058</epage><pages>1049-1058</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>Capillary electrophoresis (CE) with tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)3(2+)]-electrogenerated chemiluminescence (ECL) detection is a promising method for clinical analysis. In this study, a method combining CE with Ru(bpy)3(2+) ECL (CE-ECL) detection that can be applied to amine-containing clinical species was developed, and the performance of CE-ECL as a quantitative method for determination of sulpiride in human plasma or urine was evaluated.
Sulpiride was separated by capillary zone electrophoresis in uncoated fused-silica capillaries [50 cm x 25 microm (i.d.)] filled with phosphate buffer (pH 8.0) and a driving voltage of +15 kV, with end-column Ru(bpy)3(2+) ECL detection. A platinum disc electrode was used as working electrode. Sulpiride in human plasma or urine samples (100 microL) was extracted by a double-step liquid-liquid extraction procedure, dried under nitrogen at 35 degrees C in a water bath, and reconstituted with 100 microL of filtered water. The extraction solvent was ethyl acetate-dichloromethane (5:1 by volume).
Under optimum conditions (pH 8.0 phosphate buffer, injection for 6 s at 10 kV, and +1.2 V as detection potential), separation of sulpiride was accomplished within 4 min. The calibration curve was linear over a concentration range of 0.05-25.0 micromol/L, and the limit of detection was 2.9 x 10(-8) mol/L for sulpiride. Intra- and interday CVs for ECL intensities were <6%. Extraction recoveries of sulpiride were 95.6-101% with CVs of 2.9-6.0%. The method was clinically validated for patient plasma and urine samples.
CE combined with Ru(bpy)3(2+) ECL is reproducible, precise, selective, and enables the analysis of sulpiride in human plasma and urine. It thus is of value for rapid and efficient analysis of amine-containing analytes of clinical interest.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>12089174</pmid><doi>10.1093/clinchem/48.7.1049</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford Journals Online |
| subjects | 2,2'-Dipyridyl - analogs & derivatives 2,2'-Dipyridyl - chemistry Acetates Adult Antidepressive Agents, Second-Generation - analysis Antidepressive Agents, Second-Generation - blood Antidepressive Agents, Second-Generation - urine Antipsychotic Agents - analysis Antipsychotic Agents - blood Antipsychotic Agents - urine Biological and medical sciences Dopamine Antagonists - analysis Dopamine Antagonists - blood Dopamine Antagonists - urine Electrochemistry Electrophoresis, Capillary Humans Investigative techniques, diagnostic techniques (general aspects) Luminescent Measurements Medical sciences Methylene Chloride Middle Aged Miscellaneous. Technology Organometallic Compounds - chemistry Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Receptors, Dopamine D2 - drug effects Ruthenium Solvents Sulpiride - analysis Sulpiride - blood Sulpiride - urine |
| title | Determination of Sulpiride by Capillary Electrophoresis with End-Column Electrogenerated Chemiluminescence Detection |
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