Bone mass increase specific to the female in a line of transgenic mice overexpressing human osteoblast stimulating factor-1

We have reported that transgenic mice overexpressing human osteoblast stimulating factor-1 (osf1) under the control of the human osteocalcin promoter have a significantly higher bone mineral content and density than nontransgenic littermates. Consequently, bone mass loss due to estrogen deficiency w...

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Bibliographic Details
Published in:Journal of bone and mineral metabolism 2004-05, Vol.22 (3), p.278-282
Main Authors: HASHIMOTO-GOTOH, Tamotsu, OHNISHI, Hideo, TSUJIMURA, Atsushi, TSUNEZUKA, Hiroaki, IMAI, Kan, MASUDA, Haruchika, NAKAMURA, Toshitaka
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bone and Bones - anatomy & histology
Bone and Bones - physiology
Bone Density - physiology
Bone Development
Bones
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cytokines - genetics
Cytokines - metabolism
Diseases of the osteoarticular system
Female
Femur - anatomy & histology
Femur - physiology
Gene Expression
Genomics
Male
Medical sciences
Mice
Mice, Transgenic
Organ Size
Osteoporosis
Sex Characteristics
Vertebrates - anatomy & histology
Vertebrates - physiology
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Summary:We have reported that transgenic mice overexpressing human osteoblast stimulating factor-1 (osf1) under the control of the human osteocalcin promoter have a significantly higher bone mineral content and density than nontransgenic littermates. Consequently, bone mass loss due to estrogen deficiency was compensated for in ovariectomized female mice. Here, we show that in this transgenic line, the bone mass increase was evident in female, but not male, mice, as evaluated using the ash assay, double-emission X-ray analysis, and calcein double-labeling to determine the bone formation rate. To elucidate a possible influence on gene expression, we analyzed genomic structures of the inserted transgene and its flanking regions in mouse chromosomes. The results revealed that the transgene was integrated in the mouse repetitive sequences, 234-bp-long gamma-satellite repeats, as inverted multiple (5 + 8) copies. Twelve copies at most seemed to be functional, but no direct evidence supporting female-specific mRNA synthesis of the transgene was obtained.
ISSN:0914-8779
1435-5604
DOI:10.1007/s00774-003-0485-8