Trends in Antibacterial Susceptibility of Mastitis Pathogens During a Seven-Year Period

• Published in: Journal of dairy science 2002-05, Vol.85 (5), p.1111-1118
• Main Authors: Erskine, R.J., Walker, R.D., Bolin, C.A., Bartlett, P.C., White, D.G.
• Format: Article
• Language: English
• Subjects: Animal productions; Animals; Anti-Bacterial Agents - pharmacology; antibacterial; Bacteria - drug effects; Biological and medical sciences; Cattle; Drug Resistance, Microbial; Escherichia coli - drug effects; Escherichia coli - isolation & purification; Female; Fundamental and applied biological sciences. Psychology; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - isolation & purification; mastitis; Mastitis, Bovine - drug therapy; Mastitis, Bovine - microbiology; Microbial Sensitivity Tests; Milk - microbiology; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - isolation & purification; Serratia - drug effects; Serratia - isolation & purification; Staphylococcus aureus - drug effects; Staphylococcus aureus - isolation & purification; Streptococcus - drug effects; Streptococcus - isolation & purification; susceptibility; Terrestrial animal productions; Vertebrates
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(02)74172-6

Milk samples collected from dairy cattle suspected of having mastitis were submitted to the Microbiology Laboratory of the Animal Health Diagnostic Laboratory, Michigan State University, for bacteriologic culture. A total of 2778 isolates, from the years 1994 to 2000, were isolated, identified, and subjected to in vitro antimicrobial susceptibility testing using the disk diffusion method, in accordance with National Committee on Clinical Laboratory Standards (NCCLS) standards. Isolates included in this study were Streptococcus uberis, Streptococcus dysgalactiae, Streptococcus agalactiae, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Serratia marcesens, and Pseudomonas aeruginosa. The proportion of bacterial isolates determined to be susceptible did not change during the 7-yr period for the majority of bacterial-antibacterial interactions tested. However, analysis for linear trend in proportions determined that there were increases in the proportion of S. aureus isolates that were susceptible to ampicillin, penicillin, and erythromycin. For Strep. uberis, increases in the proportion of susceptible isolates occurred for oxacillin, sulfa-trimethoprim, gentamicin and pirlimycin, and a decrease in the proportion of susceptible isolates occurred with penicillin. For Strep. dysgalactiae, increases in the proportion of susceptible isolates occurred with erythromycin, gentamicin, sulfa-trimethoprim, and tetracycline. For Strep. agalactiae, increases in the proportion of susceptible isolates occurred with sulfa-trimethoprim. Among E. coli isolates, there was an increase in the proportion that were susceptible to ampicillin and cephalothin. Among K. pneumoniae isolates, there was an increase in the proportion that were susceptible to ceftiofur. Overall, there was no indication of increased resistance Of mastitis isolates to antibacterials that are commonly Used in dairy cattle.