Differentiation of type A, Asia1 and O foot-and-mouth disease virus variants, amplified by the same system, by sequencing of the capsid protein genes

A reverse transcription-dependent polymerase chain reaction (RT-PCR) is described that amplifies the genes encoding the capsid proteins VP1–3 of at least three evolutionary lineages each of the foot-and-mouth disease (FMD) virus types A, Asia1 and O. Most of these lineages are circulating at present...

Full description

Saved in:
Bibliographic Details
Published in:Journal of virological methods 2002-07, Vol.104 (2), p.117-123
Main Authors: Adam, K.-H, Marquardt, O
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Capsid - genetics
Capsid protein genes
Capsid Proteins
Endopeptidases - genetics
Foot-and-Mouth Disease - diagnosis
Foot-and-Mouth Disease Virus - classification
Foot-and-Mouth Disease Virus - genetics
Foot-and-Mouth Disease Virus - isolation & purification
Foot-and-mouth disease virus types A, Asia1 and O
Gene Amplification
Genetic Variation
Genome, Viral
Molecular Sequence Data
Nucleotide sequence determination
Reverse Transcriptase Polymerase Chain Reaction
RT-PCR
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A reverse transcription-dependent polymerase chain reaction (RT-PCR) is described that amplifies the genes encoding the capsid proteins VP1–3 of at least three evolutionary lineages each of the foot-and-mouth disease (FMD) virus types A, Asia1 and O. Most of these lineages are circulating at present in Asia and Africa. The method is not only suitable to confirm suspected outbreaks of FMD, but also describes the modulation of major and minor antigenic sites in the course of an epizootic by nucleotide sequence determination of the obtained RT-PCR products. Such knowledge helps to choose suitable vaccines for disease control.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(02)00041-1