Human S15a expression is upregulated by hepatitis B virus X protein

The hepatitis B virus (HBV)–encoded X antigen (HBxAg) may contribute to the development of hepatocellular carcinoma (HCC) through the upregulated expression of selected cellular genes. To identify these genes, RNAs isolated from HBxAg‐positive and ‐negative HepG2 cells were compared by PCR select cD...

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Bibliographic Details
Published in:Molecular carcinogenesis 2004-05, Vol.40 (1), p.34-46
Main Authors: Lian, Zhaorui, Liu, Jie, Li, Li, Li, Xianxing, Tufan, N. Lale Satiroglu, Wu, Meng-Chao, Wang, Hong-Yang, Arbuthnot, Patrick, Kew, Michael, Feitelson, Mark A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Blotting, Western
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - metabolism
Cell Division
Cloning, Molecular
Colony-Forming Units Assay
DNA, Complementary
Gene Expression Regulation, Neoplastic
Hepatitis B Antigens - genetics
Hepatitis B virus
hepatitis B x antigen
hepatocarcinogenesis
Humans
In Situ Hybridization
Liver - metabolism
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Mice
Mice, Nude
Mice, SCID
Molecular Sequence Data
Polymerase Chain Reaction
ribosomal protein
Ribosomal Proteins - genetics
Ribosomal Proteins - metabolism
Subtraction Technique
Trans-Activators - genetics
translation
Up-Regulation
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Summary:The hepatitis B virus (HBV)–encoded X antigen (HBxAg) may contribute to the development of hepatocellular carcinoma (HCC) through the upregulated expression of selected cellular genes. To identify these genes, RNAs isolated from HBxAg‐positive and ‐negative HepG2 cells were compared by PCR select cDNA subtraction. One gene overexpressed in HBxAg‐positive cells by Northern and Western blotting is the ribosomal protein S15a. The S15a mRNA is 535 base pairs, encoding a protein 130 amino acids long with a molecular weight of 14.3 kDa. S15a expression was upregulated in HBV‐infected livers, where it costained with HBxAg. Overexpression of S15a stimulated cell growth, colony formation in soft agar, and tumor formation in SCID mice. Hence, HBxAg upregulated the expression of S15a, the latter of which participates in the development of HCC, perhaps by altering the integrity of translation. © 2004 Wiley‐Liss, Inc.
ISSN:0899-1987
1098-2744
DOI:10.1002/mc.20012