The Role of gp130 in Cerebral Cortical Development: In Vivo Functional Analysis in a Mouse Exo Utero System

• Published in: The Journal of neuroscience 2002-07, Vol.22 (13), p.5516-5524
• Main Authors: Hatta, Toshihisa, Moriyama, Kenji, Nakashima, Kinichi, Taga, Tetsuya, Otani, Hiroki
• Format: Article
• Language: English
• Subjects: Animals; Antigens, CD - genetics; Antigens, CD - physiology; Cell Division - drug effects; Cell Movement - drug effects; Cerebral Cortex - anatomy & histology; Cerebral Cortex - cytology; Cerebral Cortex - embryology; Cerebral Ventricles - cytology; Cerebral Ventricles - drug effects; Cerebral Ventricles - embryology; Cytokine Receptor gp130; Embryo, Mammalian - cytology; Embryo, Mammalian - drug effects; Female; Growth Inhibitors - administration & dosage; Growth Inhibitors - pharmacology; Interleukin-6; Leukemia Inhibitory Factor; Lymphokines - administration & dosage; Lymphokines - pharmacology; Membrane Glycoproteins - genetics; Membrane Glycoproteins - physiology; Mice; Mice, Inbred ICR; Mice, Knockout; Microinjections; Mitosis; Neurons - drug effects; Neurons - physiology; Phenotype; Signal Transduction; Stem Cells - physiology; Telencephalon - cytology; Telencephalon - embryology; Telencephalon - metabolism
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/jneurosci.22-13-05516.2002

The role of gp130 in cerebral cortical histogenesis remains unknown. Mice lacking gp130 showed a hypoplastic cortical plate and decreased incorporation of 5-bromo-2'-deoxyuridine (BrdU) in progenitor cells of the developing cerebrum. In contrast, injection of leukemia inhibitory factor (LIF), a gp130 ligand, into the lateral cerebral ventricle of wild-type embryos exo utero induced hyperplasia of the cerebral cortex and increased the incorporation of BrdU in progenitor cells. Furthermore, chronologically controlled injection of LIF followed or preceded by BrdU revealed that gp130-mediated signals promote the progenitor cells to reenter the stem cell cycle without affecting the duration of cell cycle and enhance the migration of postmitotic neurons in the developing cerebrum.