Transglycosylation reaction of Mucor hiemalis endo-β- N-acetylglucosaminidase using sugar derivatives modified at C-1 or C-2 as oligosaccharide acceptors

Graphic We investigated the transglycosylation reaction of the recombinant endo-β- N-acetylglucosaminidase from Mucor hiemalis (Endo-M) expressed in Candida boidinii using such sugar derivatives as N-acylated d-glucosamines, C-glucosyl derivatives, and a 2-O-glycosylated disaccharide as acceptors. W...

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Bibliographic Details
Published in:Carbohydrate research 2004-05, Vol.339 (7), p.1403-1406
Main Authors: Yamanoi, Takashi, Tsutsumida, Maki, Oda, Yoshiki, Akaike, Eri, Osumi, Kenji, Yamamoto, Kenji, Fujita, Kiyotaka
Format: Article
Language:English
Subjects:
Acetylglucosamine - analogs & derivatives
Acetylglucosamine - chemistry
C-glucosyl derivative
Disaccharides - chemistry
Endo-M
Endo-β- N-acetylglucosaminidase
Glycosylation
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - chemistry
Molecular Structure
Mucor - enzymology
N-acylated d-glucosamine
Oligosaccharides - chemistry
Transglycosylation
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Summary:Graphic We investigated the transglycosylation reaction of the recombinant endo-β- N-acetylglucosaminidase from Mucor hiemalis (Endo-M) expressed in Candida boidinii using such sugar derivatives as N-acylated d-glucosamines, C-glucosyl derivatives, and a 2-O-glycosylated disaccharide as acceptors. We found that a variety of sugar derivatives modified at C-1 or C-2 could be used as acceptors for transglycosylation by Endo-M to create novel oligosaccharides.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2004.01.023