Effects of human antithymocyte globulin on acetylcholine synthesis, its release and choline acetyltransferase transcription in a human leukemic T-cell line

• Published in: Journal of neuroimmunology 2002-07, Vol.128 (1), p.1-8
• Main Authors: Fujii, Takeshi, Ushiyama, Naoki, Hosonuma, Kazuyo, Suenaga, Aya, Kawashima, Koichiro
• Format: Article
• Language: English
• Subjects: Acetylcholine; Acetylcholine - biosynthesis; Acetylcholine - metabolism; Antigens, CD7 - immunology; Antigens, CD7 - metabolism; Antigens, Surface - drug effects; Antigens, Surface - immunology; Antigens, Surface - metabolism; Antilymphocyte Serum - immunology; Antilymphocyte Serum - pharmacology; Calcium - metabolism; Calcium Signaling - drug effects; Calcium Signaling - immunology; CD11 Antigens - immunology; CD11 Antigens - metabolism; Cell Division - drug effects; Cell Division - immunology; Choline acetyltransferase; Choline O-Acetyltransferase - genetics; Choline O-Acetyltransferase - metabolism; Humans; Intracellular Fluid - drug effects; Intracellular Fluid - immunology; Intracellular Fluid - metabolism; Leukemia; RNA, Messenger - drug effects; RNA, Messenger - metabolism; Signal Transduction - drug effects; Signal Transduction - immunology; T-lymphocyte; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Transcription, Genetic - drug effects; Transcription, Genetic - immunology; Tumor Cells, Cultured; Up-Regulation - drug effects; Up-Regulation - immunology
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/S0165-5728(02)00111-X

Lymphocytes possess an independent, nonneuronal cholinergic system. In the present study, we investigated the short- and long-term effects of antithymocyte globulin (ATG)-Fresenius (ATG-F), a human antithymocyte globulin that binds to CD2, CD7 and CD11a, on acetylcholine (ACh) synthesis and transcription of choline acetyltransferase (ChAT) in CCRF-CEM cells, a human leukemic T-cell line. In the short-term (6 h), ATG-F enhanced ACh release, likely through transient increases in intracellular Ca 2+ ([Ca 2+] i) mediated by CD7, which led to declines in intracellular ACh content. By 48 h, however, the ACh content had increased as compared to control due to up-regulation of ChAT expression mediated by CD11a.