Globotriaosylceramide isoform profiles in human plasma by liquid chromatography–tandem mass spectrometry

Globotriaosylceramide (GL3) is a heterogeneous glycosphingolipid that is elevated in the blood plasma of patients diagnosed with Fabry disease. GL3 consists of numerous isoforms, some of which are distinctly specific to human plasma. An electrospray-ionization LC/MS/MS method has been developed that...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-06, Vol.805 (1), p.127-134
Main Authors: Nelson, Bryant C, Roddy, Thomas, Araghi, Shaparak, Wilkens, Dennis, Thomas, John J, Zhang, Kate, Sung, Crystal C.-C, Richards, Susan M
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, Liquid - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Globotriaosylceramide
Humans
Isomerism
Lipids
Medical sciences
Pharmacology. Drug treatments
Spectrometry, Mass, Electrospray Ionization - methods
Trihexosylceramides - blood
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Summary:Globotriaosylceramide (GL3) is a heterogeneous glycosphingolipid that is elevated in the blood plasma of patients diagnosed with Fabry disease. GL3 consists of numerous isoforms, some of which are distinctly specific to human plasma. An electrospray-ionization LC/MS/MS method has been developed that has the capacity to monitor the GL3 isoform profiles in plasma extracts. Total GL3 is extracted from human plasma via chloroform/methanol liquid–liquid extraction, purified by C 18 solid-phase extraction and analyzed by multiple reaction monitoring LC/MS/MS. The relative responses of eight selected isoforms are calculated on the basis of the total GL3 response and the isoform responses are subsequently utilized to construct isoform profile plots.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2004.02.032