The gene expression and deficiency phenotypes of Cockayne syndrome B protein in Caenorhabditis elegans

The Caenorhabditis elegans Cockayne syndrome B protein homologue is encoded by 10 exons of the predicted open reading frame F53H4.1. The gene is expressed in germ cells and all somatic cells of the embryonic to adult stage. Although the gene expression was ubiquitous, its expression level was relati...

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Bibliographic Details
Published in:FEBS letters 2002-07, Vol.522 (1), p.47-51
Main Authors: Lee, Myon Hee, Ahn, Byungchan, Choi, In Soon, Koo, Hyeon-Sook
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans
Cockayne Syndrome - genetics
CSB, Cockayne syndrome B
DNA Helicases - genetics
DNA Repair
DNA Repair Enzymes
dsRNA, double-stranded RNA
Gene Expression
Gene Silencing
GFP, green fluorescent protein
Humans
NER, nucleotide excision repair
Nucleotide excision repair
ORF, open reading frame
Phenotype
Poly-ADP-Ribose Binding Proteins
Recombinant Fusion Proteins - genetics
RNA, Double-Stranded
RNA, Small Interfering
RNA, Untranslated
RNAi, RNA interference
RT-PCR, reverse transcription-polymerase chain reaction
TCR, transcription-coupled repair
Transcription-coupled repair
UV sensitivity
XP, xeroderma pigmentosum
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Summary:The Caenorhabditis elegans Cockayne syndrome B protein homologue is encoded by 10 exons of the predicted open reading frame F53H4.1. The gene is expressed in germ cells and all somatic cells of the embryonic to adult stage. Although the gene expression was ubiquitous, its expression level was relatively higher in dividing cells and cells that play fundamental roles in essential physiological functions such as feeding, sensation, and reproduction. RNA interference of the gene hypersensitized C. elegans to UV radiation, as observed in enhanced germ cell proliferation arrest and apoptosis, and increased embryonic lethality, suggesting its role in nucleotide excision repair.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)02880-6