Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens

Late-onset infections of synthetic vascular grafts (LO-SVGIs) are generally caused by staphylococci that produce a slime polysaccharide and grow as a biofilm on the graft surface. We developed an ELISA to detect serum antibodies against staphylococcal slime polysaccharide antigens (SSPA). Patients w...

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Bibliographic Details
Published in:The Lancet (British edition) 2002-06, Vol.359 (9324), p.2166-2168
Main Authors: Selan, Laura, Passariello, Claudio, Rizzo, Luigi, Varesi, Paola, Speziale, Francesco, Renzini, Giulio, Thaller, Maria Cristina, Fiorani, Paolo, Rossolini, Gian Maria
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Bacterial - blood
Antigens
Bacterial diseases
Biofilms
Biological and medical sciences
Blood Vessel Prosthesis - microbiology
Corporate sponsorship
Data analysis
Diagnostic systems
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Graft Survival - immunology
Grafting
Grafts
Human bacterial diseases
Humans
Immunoassay
Immunoglobulin G - immunology
Immunoglobulin M
Immunoglobulin M - immunology
Immunoglobulins
Infections
Infectious diseases
Laboratories
Medical sciences
Patients
Polysaccharides
Postoperative Complications - diagnosis
Postoperative Complications - immunology
Skin & tissue grafts
Slime
Staphylococcal Infections - diagnosis
Staphylococcal Infections - immunology
Staphylococcal infections, streptococcal infections, pneumococcal infections
Staphylococcus epidermidis
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Description
Summary:Late-onset infections of synthetic vascular grafts (LO-SVGIs) are generally caused by staphylococci that produce a slime polysaccharide and grow as a biofilm on the graft surface. We developed an ELISA to detect serum antibodies against staphylococcal slime polysaccharide antigens (SSPA). Patients with an ongoing staphylococcal LO-SVGI had greater titres of IgM antibodies against SSPA than did patients in other groups. Antibody titres of 0·40 ELISA units (EU) or more, or 0·35 EU or more detected 97% and 100% of staphylococcal LO-SVGIs, respectively, 0% and 2% titre/unit false-positive results. Our findings suggest that such an ELISA represents a sensitive, specific, and non-invasive diagnostic test for staphylococcal LO-SVGIs.
ISSN:0140-6736
1474-547X
DOI:10.1016/S0140-6736(02)09086-4