An automated ‘bio-intact’ PTH assay: a step towards standardisation and improved correlation with parathyroid function in renal disease

Background: Most methods for the determination of parathyroid hormone (PTH) show cross-reactivity with N-truncated forms of PTH. The analytic and diagnostic value of a recently developed automated PTH test without this cross-reactivity was examined. Methods: The PTH levels of 73 patients undergoing...

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Bibliographic Details
Published in:Clinica chimica acta 2004-05, Vol.343 (1), p.167-171
Main Authors: Savoca, Reto, Bock, Andreas, Kraenzlin, Marius E., Schmid, Hans-Rudolf, Huber, Andreas R.
Format: Article
Language:English
Subjects:
Aged
Automation
Biomarkers - analysis
Bone and Bones - metabolism
Bone markers
Chemistry, Clinical - standards
Dialysis
Female
Humans
Intact PTH
Kidney Diseases - blood
Kidney Diseases - metabolism
Male
Parathyroid Hormone - blood
Parathyroid Hormone - metabolism
PTH
PTH fragments
Renal Dialysis
Secondary hyperparathyroidism
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Summary:Background: Most methods for the determination of parathyroid hormone (PTH) show cross-reactivity with N-truncated forms of PTH. The analytic and diagnostic value of a recently developed automated PTH test without this cross-reactivity was examined. Methods: The PTH levels of 73 patients undergoing hemodialysis were compared using the ‘bio-intact’ PTH (Nichols Institute Diagnostics) and 3 ‘intact’ PTH tests (from Nichols, Roche Elecsys® and Diagnostics Corporation DPC). Further, the (non 1–84) PTH fragment and the PTH ratio (bio-intact PTH/(non 1–84) PTH) were calculated. All results were then correlated with biochemical bone markers (bone-specific alkaline phosphatase and bone collagen C-terminal telopeptides in serum). Results: ‘Bio-intact’ PTH values were lower than the PTH results generated by the ‘intact’ PTH assays. Results of all PTH tests were closely correlated ( r=0.96–0.98, p
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cccn.2004.01.024