Recent developments in the optimization of thermostable DNA polymerases for efficient applications

Thermostable DNA polymerases have a crucial role in current methods of DNA amplification and sequencing. Major improvements in these methods have been made in recent years, largely as a result of a marked increase in our understanding of how DNA polymerases function, particularly in DNA sequencing a...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2004-05, Vol.22 (5), p.253-260
Main Authors: Pavlov, Andrey R., Pavlova, Nadejda V., Kozyavkin, Sergei A., Slesarev, Alexei I.
Format: Article
Language:English
Subjects:
Archaea - enzymology
Archaea - genetics
Archaeal Proteins - chemistry
Archaeal Proteins - genetics
Bacteria - enzymology
Bacteria - genetics
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Biological and medical sciences
Biotechnology
Deoxyribonucleotides - chemistry
DNA Polymerase I - chemistry
DNA Polymerase I - genetics
DNA Primers
DNA-Directed RNA Polymerases - chemistry
Enzyme Stability - genetics
Fundamental and applied biological sciences. Psychology
Hot Temperature
Kinetics
Methods. Procedures. Technologies
Polymerase Chain Reaction - methods
Protein engineering
Sequence Analysis, DNA - methods
Viral Proteins
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Summary:Thermostable DNA polymerases have a crucial role in current methods of DNA amplification and sequencing. Major improvements in these methods have been made in recent years, largely as a result of a marked increase in our understanding of how DNA polymerases function, particularly in DNA sequencing and the polymerase chain reaction. There is an untapped potential in genetically modified DNA polymerases, which could provide significant advantages over their natural counterparts that are used today. The development of such enzymes would have an immediate impact on the efficiency and accuracy of DNA amplification applications essential to the success of the Human Genome Initiative.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2004.02.011