PUVA-induced apoptosis involves mitochondrial dysfunction caused by the opening of the permeability transition pore

The mechanism of cell death was investigated in Jurkat cells exposed to the combination of psoralen and UVA irradiation (PUVA). Apoptosis was by far prevailing over necrosis and involved mitochondrial dysfunction. The collapse of mitochondrial membrane potential, appears to be caused by the opening...

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Bibliographic Details
Published in:FEBS letters 2002-07, Vol.522 (1), p.168-172
Main Authors: Canton, Marcella, Caffieri, Sergio, Dall’Acqua, Francesco, Di Lisa, Fabio
Format: Article
Language:English
Subjects:
Apoptosis
CsA, cyclosporin A
Cyclosporin A
FCCP, carbonyl cyanide-p-trifluoromethoxyphenyl-hydrazone
Ficusin - pharmacology
Humans
Intracellular Membranes - drug effects
Intracellular Membranes - physiology
Intracellular Membranes - radiation effects
Ion Channels - antagonists & inhibitors
Jurkat Cells
Membrane Potentials - drug effects
Membrane Potentials - physiology
Membrane Potentials - radiation effects
Mitochondria
Mitochondria - drug effects
Mitochondria - physiology
Mitochondria - radiation effects
Mitochondrial Membrane Transport Proteins
PARP, poly(ADP-ribose) polymerase
PDT, photodynamic therapy
Permeability transition
Photosensitizing Agents - pharmacology
PI, propidium iodide
Poly(ADP-ribose) Polymerases - metabolism
POP, photo-oxidized products of psoralen
Psoralen
PTP, mitochondrial permeability transition pore
PUVA, psoralen+UVA
ROS, reactive oxygen species
TMRM, tetramethylrhodamine methyl ester
Ultraviolet Rays
Δψm, mitochondrial membrane potential
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Summary:The mechanism of cell death was investigated in Jurkat cells exposed to the combination of psoralen and UVA irradiation (PUVA). Apoptosis was by far prevailing over necrosis and involved mitochondrial dysfunction. The collapse of mitochondrial membrane potential, appears to be caused by the opening of the mitochondrial permeability transition pore since its inhibitor, cyclosporin A, prevented mitochondrial dysfunction and largely attenuated apoptosis. Apoptosis also occurred in cells treated with the photoproducts generated by irradiating psoralen in vitro with an oxygen-dependent process. Thus, the involvement of reactive oxygen species in the onset of PUVA-induced apoptosis appears mostly related to psoralen photooxidation.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)02926-5