Identification of the major vanilloid component in Capsicum extract by HPLC-EC and HPLC-MS

A sensitive multi-channel HPLC-electrochemical (EC) method has been developed to determine the vanilloid content in the complex Capsicum annuum extract Capsibiol. Chromatographic separation was achieved within 10 min using a YMC Basic S5 column with a mobile phase containing chloroacetic acid, hepta...

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Bibliographic Details
Published in:Phytochemical analysis 2004-03, Vol.15 (2), p.117-120
Main Authors: Zhou, Q, Zhu, Y, Chiang, H, Yagiz, K, Morre, D.M, Janle, E, Kissinger, P.T
Format: Article
Language:English
Subjects:
capsaicin
Capsibiol
Capsicum - chemistry
Capsicum annuum
chemical analysis
chemical structure
Chromatography, High Pressure Liquid - methods
Electrochemistry - methods
high performance liquid chromatography
HPLC-EC
HPLC-MS
Mass Spectrometry - methods
Molecular Structure
phytochemicals
plant extracts
Plant Extracts - chemistry
Sensitivity and Specificity
Solanaceae
spectral analysis
Time Factors
vanillic acid
Vanillic Acid - analysis
Vanillic Acid - chemistry
vanilloid
vanilloids
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Summary:A sensitive multi-channel HPLC-electrochemical (EC) method has been developed to determine the vanilloid content in the complex Capsicum annuum extract Capsibiol. Chromatographic separation was achieved within 10 min using a YMC Basic S5 column with a mobile phase containing chloroacetic acid, heptane sulphonic acid and acetonitrile. The multi-channel detector simultaneously applied four potentials between +500 and +800 mV (referenced to a silver/silver chloride electrode) to four glassy carbon working electrodes. The most abundant (0.94 mg/g) vanilloid analogue in the Capsibiol sample demonstrated an electrochemical reactivity and retention time similar to that of vanillic acid in HPLC-EC analysis. Its identity was confirmed by HPLC-MS using a Zorbax SB-CN column with a mobile phase containing formic acid and methanol.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.754