Determination of remifentanil in human blood by capillary gas chromatography with nitrogen-selective detection

A validated method for the determination of remifentanil in human blood, applicable to all therapeutic concentrations, using capillary GC with nitrogen-specific detection and fentanyl as the internal standard has been developed. Citrated whole blood samples were extracted into 1-chlorobutane followi...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2002-07, Vol.775 (1), p.97-101
Main Authors: Bjorksten, A.R, Chan, C, Crankshaw, D.P
Format: Article
Language:English
Subjects:
Analgesics, Opioid - blood
Chromatography, Gas - methods
Humans
Nitrogen
Piperidines - blood
Remifentanil
Reproducibility of Results
Citations: Items that cite this one
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Summary:A validated method for the determination of remifentanil in human blood, applicable to all therapeutic concentrations, using capillary GC with nitrogen-specific detection and fentanyl as the internal standard has been developed. Citrated whole blood samples were extracted into 1-chlorobutane following precipitation of proteins with methanol. The drugs were back extracted into 10 m M HCl and re-extracted into methanol–1-chlorobutane. The extracts were reconstituted in methanol and injected onto a 25-m BPX-5 column. The lower limit of quantitation was 0.2 ng/ml with within- and between-day coefficients of variation of less than 15%.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(02)00178-2