Simple reversed-phase ion-pair liquid chromatography assay for the simultaneous determination of mycophenolic acid and its glucuronide metabolite in human plasma and urine

A simple and reproducible reversed-phase ion-pair high-performance liquid chromatographic (HPLC) method using isocratic elution with UV absorbance detection is presented for the simultaneous quantitation of mycophenolic acid (MPA) and MPA-glucuronide (MPAG) in human plasma and urine. The sample prep...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-06, Vol.805 (1), p.101-112
Main Authors: Yau, Wai-Ping, Vathsala, Anantharaman, Lou, Huei-Xin, Chan, Eli
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Buffers
Calibration
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Glucuronides - blood
Glucuronides - urine
Humans
Hydrogen-Ion Concentration
Medical sciences
Mycophenolic acid
Mycophenolic Acid - blood
Mycophenolic Acid - pharmacokinetics
Mycophenolic Acid - urine
Mycophenolic acid glucuronide
Pharmacology. Drug treatments
Reproducibility of Results
Sensitivity and Specificity
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Summary:A simple and reproducible reversed-phase ion-pair high-performance liquid chromatographic (HPLC) method using isocratic elution with UV absorbance detection is presented for the simultaneous quantitation of mycophenolic acid (MPA) and MPA-glucuronide (MPAG) in human plasma and urine. The sample preparation procedures involved simple protein precipitation for plasma and 10-fold dilution for urine. Each analytical run was completed within 15 min, with MPAG and MPA being eluted at 3.8 and 11.4 min, respectively. The optimized method showed good performance in terms of specificity, linearity, detection and quantitation limits, precision and accuracy. This assay was demonstrated to be applicable for clinical pharmacokinetic studies.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2004.02.020