Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

Twenty-two highly variable SSR markers were developed in Douglas-fir [ Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of...

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Published in:Theoretical and applied genetics 2004-03, Vol.108 (5), p.873-880
Main Authors: SLAVOV, G. T, HOWE, G. T, YAKOVLEV, I, EDWARDS, K. J, KRUTOVSKII, K. V, TUSKAN, G. A, CARLSON, J. E, STRAUSS, S. H, ADAMS, W. T
Format: Article
Language:English
Subjects:
Animal populations
Base Sequence
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
DNA Primers
Fundamental and applied biological sciences. Psychology
Genetic Markers
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Heterozygote
Phenotype
Polymorphism, Genetic
Pseudotsuga menziesii
Pteridophyta, spermatophyta
Trees - genetics
Vegetals
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Summary:Twenty-two highly variable SSR markers were developed in Douglas-fir [ Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-003-1490-y