Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor

We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein–protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibod...

Full description

Saved in:
Bibliographic Details
Published in:Biomolecular engineering 2004-04, Vol.21 (2), p.67-72
Main Authors: Zhong, Joan Q., Freyzon, Yelena, Ehrlich, Daniel J., Matsudaira, Paul
Format: Article
Language:English
Subjects:
Affinity fluorescent protein
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - metabolism
Binding Sites - genetics
Biosensing Techniques
Biosensor
Enhanced fluorescence intensity
Fluorescence
Green fluorescent protein
Green Fluorescent Proteins - chemistry
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Hemagglutinins - chemistry
Hemagglutinins - genetics
Hemagglutinins - metabolism
Protein Binding - genetics
Protein Engineering
Protein Structure, Tertiary
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Sensitivity and Specificity
Solid-phase assay
Spectrometry, Fluorescence
Surface Properties
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein–protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface.
ISSN:1389-0344
1878-559X
DOI:10.1016/j.bioeng.2003.10.004