Donor and recipient rat strains affect full-term development of one-cell zygotes cultured to morulae/blastocysts

The present study was conducted to examine the developmental potential to offspring of rat embryos cultured from 1-cell to morula/blastocyst stage. Pronuclear zygotes from Wistar * Wistar or (SD * DA) * Wistar strains were cultured in modified rat 1-cell embryo culture medium (mR1ECM) for 96 h in 5%...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2004, Vol.50(2), pp.191-195
Main Authors: Kato, M. (National Inst. for Physiological Sciences, Okazaki, Aichi (Japan)), Ishikawa, A, Hochi, S, Hirabayashi, M
Format: Article
Language:English
Subjects:
ANIMAL EMBRYOS
Animals
BIOLOGICAL DIFFERENCES
Blastocyst - cytology
Blastocyst - metabolism
Cell Division
Cells, Cultured
Culture Media - pharmacology
DONOR ANIMALS
EMBRYO TRANSFER
EMBRYONIC DEVELOPMENT
Female
Hybrid
HYBRIDS
IN VITRO CULTURE
Male
Morula
mR1ECM
Pregnancy
Pregnancy, Animal
Rat zygotes
RATS
Rats, Wistar
RECIPIENT ANIMALS
Species Specificity
Time Factors
Zygote - cytology
Zygote - physiology
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Summary:The present study was conducted to examine the developmental potential to offspring of rat embryos cultured from 1-cell to morula/blastocyst stage. Pronuclear zygotes from Wistar * Wistar or (SD * DA) * Wistar strains were cultured in modified rat 1-cell embryo culture medium (mR1ECM) for 96 h in 5% C02 in air at 37 C. The proportion of the 3-way cross hybrid zygotes developing into morula/blastocyst stage (74%) was higher than that of the Wistar zygotes (66%). Day-5 morulae/ blastocysts developed in vitro were transferred into Day-3 or -4 pseudopregnant recipients of Wistar or SD * DA strain. The transfer of cultured embryos resulted in the birth of offspring at 13-59%, while that of non-cultured control blastocysts showed birth rates of 35-65%. The best offspring rate of cultured embryos (59%) was obtained when the hybrid 1-cell zygotes were cultured in mR1ECM medium and transferred into the 2-days earlier uteri of SD * DA recipients. These results suggest that genetic background of recipients as well as donors is a possible factor affecting full-term development of rat morulae/blastocysts derived from 1-cell stage zygotes cultured in vitro.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.50.191