Bone Marrow‐Derived Stem Cells Can Differentiate into Retinal Cells in Injured Rat Retina

It has recently been shown that bone marrow cells can differentiate into various lineage cells including neural cells in vitro and in vivo. We therefore examined whether bone marrow stem cells can differentiate into retinal neural cells in adult rats. PKH‐67‐labeled stem cell‐enriched bone marrow ce...

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Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Ohio), 2002-01, Vol.20 (4), p.279-283
Main Authors: Tomita, Minoru, Adachi, Yasushi, Yamada, Haruhiko, Takahashi, Kanji, Kiuchi, Katsuji, Oyaizu, Haruki, Ikebukuro, Kazuya, Kaneda, Hiroyuki, Matsumura, Miyo, Ikehara, Susumu
Format: Article
Language:English
Subjects:
Amacrine Cells - cytology
Amacrine Cells - metabolism
Animals
Astrocytes - cytology
Astrocytes - metabolism
BMSCs
BMT
Calbindins
Cell Differentiation - physiology
Cell Movement - physiology
Glial Fibrillary Acidic Protein - metabolism
Graft Survival - physiology
Hematopoietic Stem Cell Transplantation - methods
Hematopoietic Stem Cell Transplantation - trends
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - metabolism
Immunohistochemistry
Leukocyte Common Antigens - metabolism
Male
Neurons - cytology
Neurons - metabolism
Rats
Retina - cytology
Retina - injuries
Retina - surgery
Retinal Diseases - therapy
Retinal Rod Photoreceptor Cells - cytology
Retinal Rod Photoreceptor Cells - metabolism
Retinal transplantation
Rhodopsin - metabolism
S100 Calcium Binding Protein G - metabolism
Vimentin - metabolism
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Summary:It has recently been shown that bone marrow cells can differentiate into various lineage cells including neural cells in vitro and in vivo. We therefore examined whether bone marrow stem cells can differentiate into retinal neural cells in adult rats. PKH‐67‐labeled stem cell‐enriched bone marrow cells (BMCs) were injected into the vitreous space of eyes in which the retinas had been mechanically injured using a hooked needle. Two weeks after the injection of these cells, immunohistochemical examinations were carried out. The stem cell‐enriched BMCs had been incorporated and had differentiated into retinal neural cells in the injured retina. The stem cell‐enriched BMCs had accumulated mainly in the outer nuclear layer around the injured sites. The incorporated cells expressed glial fibrillary acidic protein, calbindin, rhodopsin, and vimentin. These results raise the possibility that stem cell‐enriched BMCs have the ability to differentiate into retinal neural cells, and that the injection of stem cell‐enriched BMCs into the retina would help repair damaged retinal cells.
ISSN:1066-5099
1549-4918
DOI:10.1634/stemcells.20-4-279