Zyxin is up-regulated during megakaryocytic differentiation of human UT-7/c-mpl cells

To characterize genes involved in megakaryocytic commitment, we compared expression profiles of bipotent cells (UT-7/c-mpl) with those of the same cells induced to differentiate towards megakaryopoiesis in the presence of TPO. Using cDNA arrays, we showed that 12 out of 2260 genes changed their expr...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-05, Vol.318 (2), p.439-443
Main Authors: Decraene, C, Garçon, L, Lacout, C, Sabri, S, Auffray, C, Vainchenker, W, Duménil, D, Piétu, G, Svinartchuk, F
Format: Article
Language:English
Subjects:
Adhesion
Antigens, CD34 - metabolism
Blotting, Northern
cDNA arrays
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cell Line
Cytoskeletal Proteins
Differentiation
Erythroblasts - metabolism
Fluorescent Antibody Technique
Gene Expression Profiling
Glycoproteins - biosynthesis
Glycoproteins - genetics
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - drug effects
Hematopoietic Stem Cells - metabolism
Humans
Integrin
Megakaryocytes
Megakaryocytes - cytology
Megakaryocytes - metabolism
Megakaryocytes - physiology
RNA - biosynthesis
Thrombopoietin
Thrombopoietin - pharmacology
Up-Regulation - drug effects
Zyxin
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Summary:To characterize genes involved in megakaryocytic commitment, we compared expression profiles of bipotent cells (UT-7/c-mpl) with those of the same cells induced to differentiate towards megakaryopoiesis in the presence of TPO. Using cDNA arrays, we showed that 12 out of 2260 genes changed their expression level after 6 h of TPO stimulation. One of these genes encodes for zyxin, a cytoskeleton protein component. Zyxin is up-regulated at the mRNA and protein levels in UT-7/c-mpl cells in response to TPO confirming the reliability of the cDNA array technology. Similarly, when CD34 positive cells were induced to differentiate into megakaryocytes, zyxin mRNA was accumulated. Furthermore, when megakaryocytes were allowed to spread on fibrinogen, formation of stress fibers and lamellipodia was induced and zyxin was localized at the picks of actin stress fibers. These results suggest an important role for zyxin during megakaryocytic differentiation and more precisely in the regulation of the integrin mediated adhesion process in megakaryocytes.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.04.047