Transcriptional regulation of the LAT-1/CD98 light chain

LAT-1/CD98 amino acid transporter expression and activity are induced in hepatic cells deprived of arginine. The promoter dependency of this regulation was investigated. LAT-1 expression, in contrast to that of CD98 heavy chain 4F2, was actinomycin D sensitive in cells cultured without arginine. Tra...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-05, Vol.318 (2), p.529-534
Main Authors: Padbury, James F, Diah, Sri K, McGonnigal, Bethany, Miller, Carla, Fugere, Celine, Kuzniar, Magdalena, Thompson, Nancy L
Format: Article
Language:English
Subjects:
Amino acid
Animals
Arginine
Arginine - metabolism
Base Sequence
beta-Galactosidase - metabolism
CD98
Cell Line
Dactinomycin - pharmacology
Fusion Regulatory Protein 1, Light Chains - biosynthesis
Fusion Regulatory Protein 1, Light Chains - genetics
Gene Expression Regulation - drug effects
Gene Expression Regulation - genetics
Genes, Reporter
Hepatic cells
Hepatocytes - cytology
Hepatocytes - metabolism
Humans
LAT-1 promoter
Male
Molecular Sequence Data
Promoter Regions, Genetic - genetics
Rats
Rats, Inbred F344
Transcription, Genetic
Transcriptional regulation
Transfection
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Summary:LAT-1/CD98 amino acid transporter expression and activity are induced in hepatic cells deprived of arginine. The promoter dependency of this regulation was investigated. LAT-1 expression, in contrast to that of CD98 heavy chain 4F2, was actinomycin D sensitive in cells cultured without arginine. Transient transfection analysis with promoter reporter constructs including the 2 kbp LAT-1 promoter or a sub-sequence containing multiple potential amino acid response elements failed to show significant amino acid sensitivity in various cell types. Chromatin-dependency did not appear to account for this result as hepatic cell clones stably transfected with the promoter constructs showed little or no arginine or leucine responsive promoter activity. These studies suggest that while amino acid sensitivity of LAT-1 expression is transcriptionally regulated, cis elements within the proximal promoter do not directly mediate this regulation. Understanding mechanisms by which this gene responds to amino acid availability will contribute to our knowledge of how eukaryotic cells sense and respond to their environment.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.04.062