Antigen expression in biofilm cells of Aeromonas hydrophila employed in oral vaccination of fish

Total protein, S-layer protein and lipopolysaccharides (LPS) of biofilm cells of Aeromonas hydrophila were analysed by SDS–PAGE and compared with that of planktonic cells. In the whole cell lysate of biofilm cells, about 15 proteins were repressed while three new proteins were expressed compared to...

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Bibliographic Details
Published in:Fish & shellfish immunology 2004-03, Vol.16 (3), p.429-436
Main Authors: Asha, A., Nayak, D.K., Shankar, K.M., Mohan, C.V.
Format: Article
Language:English
Subjects:
Aeromonas hydrophila
Aeromonas hydrophila - chemistry
Aeromonas hydrophila - immunology
Aquaculture - methods
Bacterial Proteins - analysis
Biofilm oral vaccine
Biofilms
Electrophoresis, Polyacrylamide Gel
Lipopolysaccharides
Lipopolysaccharides - analysis
Pisces
Proteins - analysis
S-layer
S-layer protein
Total protein
Vaccination - methods
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Summary:Total protein, S-layer protein and lipopolysaccharides (LPS) of biofilm cells of Aeromonas hydrophila were analysed by SDS–PAGE and compared with that of planktonic cells. In the whole cell lysate of biofilm cells, about 15 proteins were repressed while three new proteins were expressed compared to that in planktonic cells. Interestingly, in biofilm cells the S-layer proteins were lost and LPS showed an additional high molecular weight band compared to that in planktonic cells. We propose that the change in LPS profile must have contributed to the loss of S-layer. Also, the high molecular weight band of LPS might play a role in the better performance of biofilm oral vaccine by eliciting a protective immune response.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2003.08.001