In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expression

Electropulsation is one of the non-viral methods successfully used to transfer genes into living cells in vitro as in vivo. This approach shows promise in the field of gene and cellular therapies. The present paper first describes the factors controlling electropermeabilization to small molecules (

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2004-06, Vol.33 (2), p.126-135
Main Authors: Golzio, M, Rols, M.P, Teissié, J
Format: Article
Language:English
Subjects:
Animals
Cell Membrane Permeability
Cell Survival
CHO Cells
Cricetinae
Cricetulus
DNA - genetics
Electropermeabilization
Electroporation
Electroporation - methods
Gene transfer
Gene Transfer Techniques
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Mice
Mice, Inbred BALB C
Muscle, Skeletal - metabolism
Muscle, Skeletal - ultrastructure
Transfection - methods
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Description
Summary:Electropulsation is one of the non-viral methods successfully used to transfer genes into living cells in vitro as in vivo. This approach shows promise in the field of gene and cellular therapies. The present paper first describes the factors controlling electropermeabilization to small molecules (
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2003.11.003