Identification and characterization of a novel Chlamydia trachomatis reticulate body protein

Abstract The genome of the obligate intracellular bacterium Chlamydia trachomatis comprises 894 genes predicted by computer-based analysis. As part of a large-scale proteome analysis of C. trachomatis, a small abundant protein encoded by a previously unrecognized novel 204-bp open reading frame was...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2002-07, Vol.212 (2), p.193-202
Main Authors: Shaw, Allan C., Larsen, Martin R., Roepstorff, Peter, Christiansen, Gunna, Birkelund, Svend
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteria
Bacterial diseases
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Chlamydia
Chlamydia trachomatis
Chlamydia trachomatis - chemistry
Chlamydia trachomatis - genetics
Chlamydia trachomatis - growth & development
Chlamydophila pneumoniae - chemistry
Chlamydophila pneumoniae - genetics
Developmental stages
Electrophoresis, Gel, Two-Dimensional
Experimental bacterial diseases and models
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genomes
HeLa Cells
Homology
Humans
In Vitro Techniques
Infectious diseases
Intracellular
Mass spectrometry
Mass spectroscopy
Medical sciences
Microbiology
Molecular Sequence Data
Open Reading Frames - genetics
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Protein Biosynthesis
Proteins
Proteome
Proteomics
Short open reading frame
Small protein
Two‐dimensional gel electrophoresis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The genome of the obligate intracellular bacterium Chlamydia trachomatis comprises 894 genes predicted by computer-based analysis. As part of a large-scale proteome analysis of C. trachomatis, a small abundant protein encoded by a previously unrecognized novel 204-bp open reading frame was identified by tandem mass spectrometry. No homology of this protein was observed to proteins from other organisms. The protein was conserved in C. trachomatis but not found in Chlamydia pneumoniae. Using proteomics, we show that the expression of the protein is initiated at the middle of the developmental cycle. The protein is rapidly degraded and is only present in reticulate or intermediate bodies, suggesting a possible function in the intracellular stage of C. trachomatis development. We have termed the protein ‘7-kDa reticulate body protein’.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11266.x