Determination of carnosine in feed and meat by high-performance anion-exchange chromatography with integrated pulsed amperometric detection

• Published in: Journal of Chromatography A 2004-05, Vol.1035 (2), p.285-289
• Main Authors: Nardiello, Donatella, Cataldi, Tommaso R.I
• Format: Article
• Language: English
• Subjects: Analysis; Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Animal Feed - analysis; Animals; Anion Exchange Resins; Biological and medical sciences; Carnosine; Carnosine - analysis; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Ion Exchange - methods; Electrochemistry - methods; Exact sciences and technology; Food analysis; Food industries; Fundamental and applied biological sciences. Psychology; General pharmacology; Meat - analysis; Meat and meat product industries; Medical sciences; Pharmacology. Drug treatments; Pulsed amperometric detection; Sensitivity and Specificity
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.02.066

Carnosine (β-alanyl- l-histidine) is a dipeptide regarded as an important molecular marker of the presence of processed animal proteins including meat and bone meal in animal feed. For its identification and quantification a sensitive and selective method by high-performance anion-exchange chromatography coupled with integrated pulsed amperometric detection (HPAEC–IPAD) was developed. The assay is based on isocratic elution with 100 mM NaOH as the mobile phase. Interferences of real matrices were efficiently removed; carnosine could be determined at the concentration ranges 0.1–100 μM with a rather low detection limit of 0.23 ng. Unlike feeds for dogs and cats, no carnosine peak was observed in all examined feeds for ruminants. The good analytical characteristics allowed carnosine determination down to 5 μg/g of feed.