Evaluation of DNA polymorphisms amplified by arbitrary primers (RAPD) as genetically associated elements to differentiate virulent and non-virulent Paracoccidioides brasiliensis isolates

Abstract Randomly amplified polymorphic DNA (RAPD) analysis of 35 Paracoccidioides brasiliensis isolates was carried out to evaluate the correlation of RAPD profiles with the virulence degree or the type of the clinical manifestations of human paracoccidioidomycosis. The dendrogram presented two mai...

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Published in:FEMS immunology and medical microbiology 2002-07, Vol.33 (3), p.151-157
Main Authors: Motta, Teresa R., Moreira-Filho, Carlos A., Mendes, Rinaldo P., Souza, Lenice R., Sugizak, Maria F., Baueb, Sonia, Calich, Vera L.G., Vaz, Celideia A.C.
Format: Article
Language:English
Subjects:
Amplification
Animals
Arbitrary primers
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Deoxyribonucleic acid
DNA
DNA polymorphism
DNA Primers
DNA, Fungal - analysis
DNA, Fungal - genetics
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Humans
Mice
Paracoccidioides - genetics
Paracoccidioides - pathogenicity
Paracoccidioides brasiliensis
Paracoccidioidomycosis - microbiology
Paracoccidioidomycosis - physiopathology
Polymorphism, Genetic
Random Amplified Polymorphic DNA Technique
Randomly amplified polymorphic DNA
Similarity
South American blastomycosis
Thallophyta, bryophyta
Vegetals
Virulence
Virulence - genetics
Virulent isolates
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Summary:Abstract Randomly amplified polymorphic DNA (RAPD) analysis of 35 Paracoccidioides brasiliensis isolates was carried out to evaluate the correlation of RAPD profiles with the virulence degree or the type of the clinical manifestations of human paracoccidioidomycosis. The dendrogram presented two main groups sharing 64% genetic similarity. Group A included two isolates from patients with chronic paracoccidioidomycosis; group B comprised the following isolates showing 65% similarity: two non-virulent, six attenuated, five virulent, eight from patients with chronic paracoccidioidomycosis and two from patients with acute paracoccidioidomycosis. The virulent Pb18 isolate and six attenuated or non-virulent samples derived from it were genetically indistinguishable (100% of similarity). Thus, in our study, RAPD patterns could not discriminate among 35 P. brasiliensis isolates according to their differences either in the degree of virulence or in the type of the clinical manifestation of this fungal infection.
ISSN:0928-8244
1574-695X
2049-632X
DOI:10.1111/j.1574-695X.2002.tb00585.x