Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method

In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to t...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2002-07, Vol.373 (4-5), p.222-226
Main Authors: Masadome, Takashi, Asano, Yasukazu, Imato, Toshihiko, Ohkubo, Satoshi, Tobita, Tatsuya, Tabei, Hisao, Iwasaki, Yuzuru, Niwa, Osamu, Fushinuki, Yoshito
Format: Article
Language:English
Subjects:
Antibodies - analysis
Antigen-Antibody Reactions
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Calibration
Equipment Design
Glass
Humans
Matching
Polymeric films
Polyvinyl Chloride - chemistry
Polyvinyl chlorides
Prisms
Refractive index
Refractivity
Refractometry
Sensors
Serum Albumin - immunology
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
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Summary:In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-002-1329-4