Online analysis of volatile organic compound emissions from Sitka spruce (Picea sitchensis)

• Published in: Tree physiology 2004-07, Vol.24 (7), p.721-728
• Main Authors: Hayward, S, Tani, A, Owen, S.M, Hewitt, C.N
• Format: Article
• Language: English
• Subjects: acetaldehyde; Acetaldehyde - metabolism; Butadienes - metabolism; computer analysis; equations; forest trees; gas emissions; Hemiterpenes - metabolism; isoprene; isoprenoids; Light; light intensity; Mass Spectrometry; mathematical models; methanol; Methanol - metabolism; Monoterpenes - metabolism; monoterpenoids; Pentanes - metabolism; Picea - physiology; Picea sitchensis; proton transfer reaction-mass spectrometry; protons; quantitative analysis; Temperature; tree growth; Trees - physiology; volatile compounds
• ISSN: 0829-318X; 1758-4469
• DOI: 10.1093/treephys/24.7.721

Volatile organic compound (VOC) emissions from Sitka spruce (Picea sitchensis Bong.) growing in a range of controlled light and temperature regimes were monitored online with a proton transfer reaction-mass spectrometer (PTR-MS) operating at a temporal resolution of ~1 min. Isoprene emissions accounted for an average of more than 70% of measured VOCs and up to 3.5% of assimilated carbon. Emission rates (E) for isoprene correlated closely with photosynthetic photon flux (PPF) and temperature, showing saturation at a PPF of between 300 and 400 micromol m-2 s-1 and a maximum between 35 and 38 °C. Under standard conditions of 30 °C and 1000 micromol m-2 s-1 PPF, the mean isoprene E was 13 microgram gdm-1 h-1, considerably higher than previously observed in this species. Mean E for acetaldehyde, methanol and monoterpenes at 30 °C were 0.37, 0.78 and 2.97 microgram gdm-1 h-1, respectively. In response to a sudden light to dark transition, isoprene E decreased exponentially by > 98% over about 3 h; however, during the first 7 min, this otherwise steady decay was temporarily but immediately depressed to ~40% of the pre-darkness rate, before rallying during the following 7 min to rejoin the general downward trajectory of the exponential decay. The sudden sharp fall in isoprene E was mirrored by a burst in acetaldehyde E. The acetaldehyde E maximum coincided with the isoprene E minimum (7 min post-illumination), and ceased when isoprene emissions resumed their exponential decay. The causes of, and linkages between, these phenomena were investigated.