Inducing precocious anaphase in cultured mammalian cells

The spindle checkpoint, which prevents anaphase onset upon spindle damage or incorrect chromosome alignment, presents a problem for experimental analysis of protein function in anaphase and cytokinesis. This is because the functional disruption of many proteins before anaphase onset can activate thi...

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Bibliographic Details
Published in:Cell motility and the cytoskeleton 2002-06, Vol.52 (2), p.61-65
Main Authors: Canman, Julie C., Salmon, E.D., Fang, Guowei
Format: Article
Language:English
Subjects:
Anaphase
Animals
Calcium-Binding Proteins - genetics
Carrier Proteins
Cell Cycle Proteins
Cells, Cultured
Fungal Proteins - genetics
Mammals - anatomy & histology
Mammals - metabolism
Microinjections
Models, Genetic
Nuclear Proteins
Phosphoproteins - genetics
Repressor Proteins - genetics
Spindle Apparatus - genetics
Spindle Apparatus - metabolism
Time Factors
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Summary:The spindle checkpoint, which prevents anaphase onset upon spindle damage or incorrect chromosome alignment, presents a problem for experimental analysis of protein function in anaphase and cytokinesis. This is because the functional disruption of many proteins before anaphase onset can activate this checkpoint, preventing anaphase and subsequent cell cycle events. This paper compares new and old methods of overriding the spindle checkpoint in prometaphase mammalian tissue culture cells. Cell Motil. Cytoskeleton 52:61–65, 2002. © 2002 Wiley‐Liss, Inc.
ISSN:0886-1544
1097-0169
DOI:10.1002/cm.10032