Human aminopeptidase B (rnpep) on chromosome 1q32.2: complementary DNA, genomic structure and expression

Aminopeptidase B (APB) is a Zn(2+)-metalloexopeptidase, which selectively removes Arg and/or Lys residues from the N-terminus of several peptide substrates. Several data strongly support the hypothesis that this enzyme could participate in the final stages of precursor processing mechanisms and/or i...

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Bibliographic Details
Published in:Gene 2002-06, Vol.292 (1-2), p.129-140
Main Authors: Piesse, Christophe, Tymms, Martin, Garrafa, Emirena, Gouzy, Cécile, Lacasa, Michel, Cadel, Sandrine, Cohen, Paul, Foulon, Thierry
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Aminopeptidases - genetics
Animals
Base Sequence
Caco-2 Cells
Chromosomes, Human, Pair 1 - genetics
DNA, Complementary - chemistry
DNA, Complementary - genetics
Epoxide Hydrolases - genetics
Female
Gene Expression
Genes - genetics
HL-60 Cells
Humans
K562 Cells
Male
Mice
Molecular Sequence Data
Promoter Regions, Genetic - genetics
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tumor Cells, Cultured
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Summary:Aminopeptidase B (APB) is a Zn(2+)-metalloexopeptidase, which selectively removes Arg and/or Lys residues from the N-terminus of several peptide substrates. Several data strongly support the hypothesis that this enzyme could participate in the final stages of precursor processing mechanisms and/or in particular inflammatory processes and tumor developments. Therefore, we have cloned the complementary DNA encoding the human APB, a 658-residues protein, containing the canonical "HEXXH(X(18))E", a signature allowing its classification in the M1 family of metallopeptidases. The genomic structure of the human APB gene (rnpep; 1q32.1-q32.2) was also determined. rnpep is bracketed by pre-protein translocase of the inner mitochondrial membrane gene and ETS family transcription factor ELF3 gene. It spans more than 24 kbp and contains 11 exons ranging from 109 to 574 bp. Finally, expression of the human APB messenger RNA (mRNA) was investigated using a pre-made dot-blot. This mRNA seems to be ubiquitous although its expression level varies depending of the cells or tissues considered.
ISSN:0378-1119
DOI:10.1016/S0378-1119(02)00650-9