Early effects of diabetes on inducible nitric oxide synthase in the kidney

NO may be responsible for the glomerular hyperfiltration observed in diabetic kidney by inducing vasodilation of the afferent arteriole. The aim of this study was to evaluate which isoform of nitric oxide synthase (NOS) is responsible for increased renal production of NO in diabetic kidney. Thirty m...

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Bibliographic Details
Published in:Acta diabetologica 2002-06, Vol.39 (2), p.91-96
Main Authors: COSENZI, A, BERNOBICH, E, BONAVITA, M, TREVISAN, R, BELLINI, G, CAMPANACCI, L
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Diabetes Mellitus, Experimental - enzymology
Diabetes Mellitus, Experimental - urine
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Immunohistochemistry
Kidney - enzymology
Male
Medical sciences
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase - urine
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Rats
Rats, Inbred WKY
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Summary:NO may be responsible for the glomerular hyperfiltration observed in diabetic kidney by inducing vasodilation of the afferent arteriole. The aim of this study was to evaluate which isoform of nitric oxide synthase (NOS) is responsible for increased renal production of NO in diabetic kidney. Thirty male WKY rats were divided into 6 groups. Five rats were sacrificed immediately, five after 20 days. In the other rats, diabetes was induced by streptozotocin. The four diabetic groups were sacrificed respectively after 5, 10, 15 and 20 days. Urine excretion of NO metabolites was assayed; immunochemistry showed the presence of inducible (iNOS) and endothelial constitutive (ecNOS) synthases in the kidney. Urinary excretion of NO metabolites increased significantly in diabetic rats five days after the induction of diabetes and at the end of the study whereas it was unchanged in the control group. Renal ecNOS remained unchanged throughout the study in all rats whereas iNOS increased significantly in diabetic rats from the fifth day until the end of the study. The results demonstrate that iNOS is activated in the kidney of rats, soon after the induction of diabetes, thus suggesting its involvement in the increased production of NO observed immediately after the onset of diabetes.
ISSN:0940-5429
1432-5233
DOI:10.1007/s005920200019