Diversity of genetic environment of bla(CTX-M) genes

Twenty-four non-clonally related enterobacterial isolates producing the emerging CTX-M-type extended-spectrum beta-lactamases were recovered from several countries including France, India, Poland, and Turkey. They had been isolated from 2000 to 2003. beta-Lactamases CTX-M-2, CTX-M-3, CTX-M-10, CTX-M...

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Bibliographic Details
Published in:FEMS microbiology letters 2004-05, Vol.234 (2), p.201-207
Main Authors: Lartigue, Marie-Frédérique, Poirel, Laurent, Nordmann, Patrice
Format: Article
Language:English
Subjects:
Base Sequence
beta-Lactamases - genetics
Cloning, Molecular
DNA Primers
Enterobacter - enzymology
Enterobacter - genetics
Enterobacter - isolation & purification
Genetic Variation
Humans
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Summary:Twenty-four non-clonally related enterobacterial isolates producing the emerging CTX-M-type extended-spectrum beta-lactamases were recovered from several countries including France, India, Poland, and Turkey. They had been isolated from 2000 to 2003. beta-Lactamases CTX-M-2, CTX-M-3, CTX-M-10, CTX-M-14 and CTX-M-15 were identified. Most of the isolates produced beta-lactamase CTX-M-15. Insertion sequence ISEcp1 was found upstream of bla(CTX-M-3), bla(CTX-M-10), bla(CTX-M-14) and bla(CTX-M-15) genes. A sequence similar to the inverted right repeat of ISEcp1 was identified downstream of bla(CTX-M-3), bla(CTX-M-10) and bla(CTX-M-15) genes suggesting the mobilization of these beta-lactamase genes by transposition events. In addition, Orf513 was identified upstream of the bla(CTX-M-2) gene. This work further underlined widespread of bla(CTX-M-15) gene associated with ISEcp1.
ISSN:0378-1097
DOI:10.1016/j.femsle.2004.01.051