Normal human epidermal keratinocytes treated with 7-dehydrocholesterol express increased levels of heat shock protein

Human skin, and its isolated cells, respond to insults with a variety of repair and protective mechanisms. One such mechanism is the production of heat shock proteins (HSPs). Heat shock proteins help the other cellular proteins fold correctly into their active three-dimensional structures. Therefore...

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Bibliographic Details
Published in:Journal of cosmetic science 2004-03, Vol.55 (2), p.149-155
Main Authors: MAMMONE, Thomas, MUIZZUDDIN, Neelam, GOYARTS, Earl, GAN, David, GIACOMONI, Paolo, MARENUS, Ken, MAES, Daniel
Format: Article
Language:English
Subjects:
Applied sciences
Cells, Cultured
Chemical industry and chemicals
Cosmetics, toiletries
Dehydrocholesterols - pharmacology
Epidermis - cytology
Epidermis - drug effects
Epidermis - metabolism
Exact sciences and technology
Heat-Shock Proteins - metabolism
Humans
Keratinocytes - drug effects
Keratinocytes - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Washing products. Cosmetics and toiletries. Perfumes
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Summary:Human skin, and its isolated cells, respond to insults with a variety of repair and protective mechanisms. One such mechanism is the production of heat shock proteins (HSPs). Heat shock proteins help the other cellular proteins fold correctly into their active three-dimensional structures. Therefore, they can enhance the survival of cells under harsh, denaturing conditions. In order to develop a means of promoting the heat shock response to prepare the skin to withstand insult, we are investigating materials that appear to protect the skin biologically. One such material is vitamin D3 and its precursors. We have observed that keratinocytes treated with 7-dehydrocholesterol (7-DHC), a precursor of vitamin D3, have increased levels of protein and mRNA for heat shock proteins. In addition, we observed that topically applied 7-DHC increases the minimal dose of UVB required to induce erythema. These data suggest that 7-DHC can induce heat shock proteins in skin keratinocytes and that they will be more resistant to UVB insult.
ISSN:1525-7886
2689-5153