Polarized Increase of Calcium and Nucleokinesis in Tangentially Migrating Neurons

Cortical interneurons originate from the ganglionic eminences and reach their final position in the cortex via tangential migratory routes. The mechanisms of this migration are poorly understood. Here we have performed confocal time-lapse analysis of cell movement in the intermediate zone of embryon...

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Bibliographic Details
Published in:Cerebral cortex (New York, N.Y. 1991) N.Y. 1991), 2004-06, Vol.14 (6), p.610-618
Main Authors: Moya, Fernando, Valdeolmillos, Miguel
Format: Article
Language:English
Subjects:
Animals
Calcium - physiology
Calcium Signaling - physiology
calcium signalling
Cell Movement - physiology
Cell Nucleus - physiology
Cell Nucleus - ultrastructure
Cells, Cultured
Cerebral Cortex - cytology
Cerebral Cortex - embryology
Cerebral Cortex - physiology
cytoskeleton
gabaergic neurons
Kinesis - physiology
Mice
Mice, Inbred C57BL
neuronal migration
Neurons - cytology
Neurons - physiology
γ-tubulin
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Summary:Cortical interneurons originate from the ganglionic eminences and reach their final position in the cortex via tangential migratory routes. The mechanisms of this migration are poorly understood. Here we have performed confocal time-lapse analysis of cell movement in the intermediate zone of embryonic mouse cortical slices in order to directly visualize their mode of migration. Tangentially migrating neurons moved by nucleokinesis, characterized by active phases of discontinuous advances of the nucleus followed by periods of quiescence. Dissociated cells from the ganglionic eminences also showed nucleokinesis associated with an increase of intracellular calcium, [Ca2+]i Calcium elevation was greatest in the proximal region of the leading process, a zone with a wide distribution of γ-tubulin. General increases in [Ca2+]i elicited by microperfussion with neurotransmitters did not elicit nucleokinesis. These results show that tangential migration uses nucleokinesis, a cell-intrinsic process in which calcium signalling is local, directional and highly regulated.
ISSN:1047-3211
1460-2199
DOI:10.1093/cercor/bhh022