Chemical and Biological Characterization of Cinnamic Acid Derivatives from Cell Cultures of Lavender (Lavandula officinalis) Induced by Stress and Jasmonic Acid

Cell cultures of lavender (Lavandula officinalis) were analyzed for the metabolite profile under normal growth conditions and under stress as well as after jasmonic acid treatment. The main compound synthesized was rosmarinic acid, which was also secreted into the culture medium. Different solvent e...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2004-05, Vol.52 (10), p.2915-2923
Main Authors: Nitzsche, Astrid, Tokalov, Sergey V, Gutzeit, Herwig O, Ludwig-Müller, Jutta
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
anaerobic conditions
anticarcinogenic activity
antioxidant activity
Antioxidants - analysis
Biological and medical sciences
biosynthesis
caffeic acid
Caffeic Acids - analysis
Caffeic Acids - metabolism
cell culture
cell lines
Cells, Cultured
Cinnamates - analysis
Cinnamates - chemistry
Cinnamates - metabolism
Culture Media, Conditioned
Cyclopentanes - pharmacology
cytotoxicity
Depsides
Economic plant physiology
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Hydrogen-Ion Concentration
jasmonic acid
Lavandula - metabolism
Lavandula angustifolia
leukemia
medicinal plants
Nitrogen metabolism and other ones (excepting carbon metabolism)
Nutrition. Photosynthesis. Respiration. Metabolism
Oxygen - administration & dosage
Oxylipins
Rosmarinic Acid
secondary metabolites
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Summary:Cell cultures of lavender (Lavandula officinalis) were analyzed for the metabolite profile under normal growth conditions and under stress as well as after jasmonic acid treatment. The main compound synthesized was rosmarinic acid, which was also secreted into the culture medium. Different solvent extraction methods at different pH values altered the profile slightly. Anoxic stress induced the synthesis of a cinnamic acid derivative, which was identified as caffeic acid by gas chromatography−mass spectrometry. Caffeic acid was also induced after treatment of the cell cultures with jasmonic acid. Although the antioxidative activity of both compounds, rosmarinic acid and caffeic acid, was confirmed in an assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH), it was demonstrated that both substances have a low cytotoxic potential in vitro using acute myeloid leukemia (HL-60) cells. The potential of the system for finding new bioactive compounds is discussed. Keywords: Caffeic acid; cinnamic acid derivatives; elicitation; HL-60 cells; jasmonic acid; Lavandula officinalis; rosmarinic acid; stress
ISSN:0021-8561
1520-5118
DOI:10.1021/jf030747j