Identification of Francisella tularensis using real-time fluorescence polymerase chain reaction

A Francisella tularensis-specific, TaqMan probe-based, real-time fluorescence polymerase chain reaction (PCR) assay required approximately 60 minutes and consistently achieved a sensitivity of < or = 10 fg of F. tularensis genomic DNA (five genome equivalents). Specificity testing against a genom...

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Bibliographic Details
Published in:Military medicine 2004-04, Vol.169 (4), p.330-333
Main Authors: McAvin, James C, Morton, Marlana M, Roudabush, Robert M, Atchley, Daniel H, Hickman, John R
Format: Article
Language:English
Subjects:
Animals
Francisella tularensis - genetics
Francisella tularensis - isolation & purification
Humans
In Vitro Techniques
Military Medicine - methods
Polymerase Chain Reaction
Sensitivity and Specificity
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Summary:A Francisella tularensis-specific, TaqMan probe-based, real-time fluorescence polymerase chain reaction (PCR) assay required approximately 60 minutes and consistently achieved a sensitivity of < or = 10 fg of F. tularensis genomic DNA (five genome equivalents). Specificity testing against a genomic DNA cross-reaction panel comprised of 22 bacterial organisms representing closely related species, diverse genera, and human genomic DNA resulted in no false positives of significance. The assay was conducted on a field-deployable thermocycler, the R.A.P.I.D. ("Ruggedized" Advanced Pathogen Identification Device), a microbial identification system that can provide rapid and accurate identification F. tularensis.
ISSN:0026-4075
1930-613X
DOI:10.7205/MILMED.169.4.330