Amperometric acetylcholine sensor catalyzed by nickel anode electrode

An amperometric method was using a nickel catalytic electrode in aqueous base solution for detecting acetylcholine (ACh). A sensing mechanism was developed in which ACh was hydrolyzed in base aqueous solution to produce the acetic anion and choline. The alcohol group of choline was oxidized to the c...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2004-07, Vol.20 (1), p.9-14
Main Authors: Lin, Shin, Liu, Chung-Chiun, Chou, Tse-Chuan
Format: Article
Language:English
Subjects:
Acetylcholine
Acetylcholine - analysis
Acetylcholine - chemistry
Amperometry
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Complex Mixtures - analysis
Complex Mixtures - chemistry
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Methods. Procedures. Technologies
Neurotransmitter Agents - analysis
Neurotransmitter Agents - chemistry
Nickel
Nickel - chemistry
Oxidation-Reduction
Reproducibility of Results
SECM
Sensitivity and Specificity
Sensor
Surface Properties
Various methods and equipments
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Summary:An amperometric method was using a nickel catalytic electrode in aqueous base solution for detecting acetylcholine (ACh). A sensing mechanism was developed in which ACh was hydrolyzed in base aqueous solution to produce the acetic anion and choline. The alcohol group of choline was oxidized to the corresponding carboxylic acid by Ni(OH) 2/NiOOH catalytic system. The amperometric response resulted from the current generated by ACh oxidation in response to step changes in ACh concentration. The potential window of limiting current of ACh anodic oxidation at the Ni interface was determined in NaOH electrolyte. The effect of NaOH electrolyte concentration on sensitivity was also discussed. At the optimum operating condition, the method exhibits a good linear relationship between the response current and the ACh concentration. The response time of the ACh sensing system was 10 s. Scanning electrochemical microscopy (SECM) with platinum micro-tips was used to investigate the diffusion layer thickness of Ni electrode.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2004.01.018