Direct Optical Detection of Singlet Oxygen from a Single Cell

Singlet oxygen has been detected in single nerve cells by its weak 1270 nm phosphorescence (a1Δg→X3Σg−) upon irradiation of a photosensitizer incorporated in the cell. Thus, one can now consider the application of direct optical imaging techniques to mechanistic studies of singlet oxygen at the sing...

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Bibliographic Details
Published in:Photochemistry and photobiology 2004-04, Vol.79 (4), p.319-322
Main Authors: Zebger, Ingo, Snyder, John W., Andersen, Lars Klembt, Poulsen, Lars, Gao, Zhan, Lambert, John D. C., Kristiansen, Uffe, Ogilby, Peter R.
Format: Article
Language:English
Subjects:
Animals
Hippocampus - chemistry
Luminescence
Mathematics
Neurons - chemistry
Photochemistry
Photosensitizing Agents - chemistry
Porphyrins - chemistry
RAPID COMMUNICATION
Rats
Rats, Wistar
Singlet Oxygen - analysis
Spectrophotometry - methods
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Summary:Singlet oxygen has been detected in single nerve cells by its weak 1270 nm phosphorescence (a1Δg→X3Σg−) upon irradiation of a photosensitizer incorporated in the cell. Thus, one can now consider the application of direct optical imaging techniques to mechanistic studies of singlet oxygen at the single-cell level.
ISSN:0031-8655
1751-1097
DOI:10.1562/RC-065R.1