Abnormalities in chromosome 17 and p53 in lung carcinoma cells detected by fluorescence in situ hybridization

The value of fluorescence in situ hybridization (FISH) as an aid to deciding the prognosis for lung carcinoma patients, comparing quantitatively the signal from the p53 gene (17p13.1) on chromosome 17, was studied. A dual‐labeling technique was used, using probes for the centromeric region of chromo...

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Published in:Pathology international 2004-06, Vol.54 (6), p.413-419
Main Authors: Kawai, Toshiaki, Hiroi, Sadayuki, Nakanishi, Kuniaki, Sakurai, Yutaka, Torikata, Chikao
Format: Article
Language:English
Subjects:
Carcinoma - genetics
Carcinoma - pathology
Carcinoma - surgery
Cell Nucleus - genetics
Cell Nucleus - pathology
chromosome
Chromosome Aberrations
Chromosomes, Human, Pair 17
Female
fluorescence in situ hybridization
Genes, p53
Humans
immunohistochemistry
In Situ Hybridization, Fluorescence - methods
Interphase
lung carcinoma
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Lung Neoplasms - surgery
Male
Middle Aged
Neoplasm Staging
p53
Prognosis
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Summary:The value of fluorescence in situ hybridization (FISH) as an aid to deciding the prognosis for lung carcinoma patients, comparing quantitatively the signal from the p53 gene (17p13.1) on chromosome 17, was studied. A dual‐labeling technique was used, using probes for the centromeric region of chromosome 17 and for the p53 gene locus. FISH was used on frozen sections of 68 surgically resected lung carcinoma (20 adenocarcinoma; 37 squamous cell carcinoma; 11 large, small, and other cell carcinoma). Hybridization signals were counted for 100–200 interphase nuclei per specimen using a Zeiss confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a bandpass filter for diaminophenolindole and a longpass filter for rhodamine. Clinicopathologic data were evaluated using the Statistical Analysis System. Chromosome 17 polysomy (three or more signals) was greater in poorly differentiated than in well‐differentiated lung carcinoma (P 
ISSN:1320-5463
1440-1827
DOI:10.1111/j.1440-1827.2004.01635.x