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Abnormalities in chromosome 17 and p53 in lung carcinoma cells detected by fluorescence in situ hybridization
The value of fluorescence in situ hybridization (FISH) as an aid to deciding the prognosis for lung carcinoma patients, comparing quantitatively the signal from the p53 gene (17p13.1) on chromosome 17, was studied. A dual‐labeling technique was used, using probes for the centromeric region of chromo...
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| Published in: | Pathology international 2004-06, Vol.54 (6), p.413-419 |
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| container_title | Pathology international |
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| creator | Kawai, Toshiaki Hiroi, Sadayuki Nakanishi, Kuniaki Sakurai, Yutaka Torikata, Chikao |
| description | The value of fluorescence in situ hybridization (FISH) as an aid to deciding the prognosis for lung carcinoma patients, comparing quantitatively the signal from the p53 gene (17p13.1) on chromosome 17, was studied. A dual‐labeling technique was used, using probes for the centromeric region of chromosome 17 and for the p53 gene locus. FISH was used on frozen sections of 68 surgically resected lung carcinoma (20 adenocarcinoma; 37 squamous cell carcinoma; 11 large, small, and other cell carcinoma). Hybridization signals were counted for 100–200 interphase nuclei per specimen using a Zeiss confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a bandpass filter for diaminophenolindole and a longpass filter for rhodamine. Clinicopathologic data were evaluated using the Statistical Analysis System. Chromosome 17 polysomy (three or more signals) was greater in poorly differentiated than in well‐differentiated lung carcinoma (P |
| doi_str_mv | 10.1111/j.1440-1827.2004.01635.x |
| format | article |
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A dual‐labeling technique was used, using probes for the centromeric region of chromosome 17 and for the p53 gene locus. FISH was used on frozen sections of 68 surgically resected lung carcinoma (20 adenocarcinoma; 37 squamous cell carcinoma; 11 large, small, and other cell carcinoma). Hybridization signals were counted for 100–200 interphase nuclei per specimen using a Zeiss confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a bandpass filter for diaminophenolindole and a longpass filter for rhodamine. Clinicopathologic data were evaluated using the Statistical Analysis System. Chromosome 17 polysomy (three or more signals) was greater in poorly differentiated than in well‐differentiated lung carcinoma (P < 0.05). p53 deletion correlated with p53 immunostaining (P < 0.05). Thus, analysis by FISH using DNA probes for chromosome 17 and p53 may be of some, albeit limited, value in determination of prognosis.</description><identifier>ISSN: 1320-5463</identifier><identifier>EISSN: 1440-1827</identifier><identifier>DOI: 10.1111/j.1440-1827.2004.01635.x</identifier><identifier>PMID: 15144400</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Science Pty</publisher><subject>Carcinoma - genetics ; Carcinoma - pathology ; Carcinoma - surgery ; Cell Nucleus - genetics ; Cell Nucleus - pathology ; chromosome ; Chromosome Aberrations ; Chromosomes, Human, Pair 17 ; Female ; fluorescence in situ hybridization ; Genes, p53 ; Humans ; immunohistochemistry ; In Situ Hybridization, Fluorescence - methods ; Interphase ; lung carcinoma ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Lung Neoplasms - surgery ; Male ; Middle Aged ; Neoplasm Staging ; p53 ; Prognosis</subject><ispartof>Pathology international, 2004-06, Vol.54 (6), p.413-419</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4565-5af601bb1ed723f3ff2929ab90a4b07579e2ba8e4bc0ac41de40f5dd589d65bb3</citedby><cites>FETCH-LOGICAL-c4565-5af601bb1ed723f3ff2929ab90a4b07579e2ba8e4bc0ac41de40f5dd589d65bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15144400$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawai, Toshiaki</creatorcontrib><creatorcontrib>Hiroi, Sadayuki</creatorcontrib><creatorcontrib>Nakanishi, Kuniaki</creatorcontrib><creatorcontrib>Sakurai, Yutaka</creatorcontrib><creatorcontrib>Torikata, Chikao</creatorcontrib><title>Abnormalities in chromosome 17 and p53 in lung carcinoma cells detected by fluorescence in situ hybridization</title><title>Pathology international</title><addtitle>Pathol Int</addtitle><description>The value of fluorescence in situ hybridization (FISH) as an aid to deciding the prognosis for lung carcinoma patients, comparing quantitatively the signal from the p53 gene (17p13.1) on chromosome 17, was studied. A dual‐labeling technique was used, using probes for the centromeric region of chromosome 17 and for the p53 gene locus. FISH was used on frozen sections of 68 surgically resected lung carcinoma (20 adenocarcinoma; 37 squamous cell carcinoma; 11 large, small, and other cell carcinoma). Hybridization signals were counted for 100–200 interphase nuclei per specimen using a Zeiss confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a bandpass filter for diaminophenolindole and a longpass filter for rhodamine. Clinicopathologic data were evaluated using the Statistical Analysis System. Chromosome 17 polysomy (three or more signals) was greater in poorly differentiated than in well‐differentiated lung carcinoma (P < 0.05). p53 deletion correlated with p53 immunostaining (P < 0.05). Thus, analysis by FISH using DNA probes for chromosome 17 and p53 may be of some, albeit limited, value in determination of prognosis.</description><subject>Carcinoma - genetics</subject><subject>Carcinoma - pathology</subject><subject>Carcinoma - surgery</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - pathology</subject><subject>chromosome</subject><subject>Chromosome Aberrations</subject><subject>Chromosomes, Human, Pair 17</subject><subject>Female</subject><subject>fluorescence in situ hybridization</subject><subject>Genes, p53</subject><subject>Humans</subject><subject>immunohistochemistry</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Interphase</subject><subject>lung carcinoma</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>Lung Neoplasms - surgery</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>p53</subject><subject>Prognosis</subject><issn>1320-5463</issn><issn>1440-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqNkE1v1DAQhi1ERT_oX0A-cUvqzzg5cKgqKJW27R5ASL1Y_phQL0m82InY5deTsKtyZS4z0rzP2HoQwpSUdK6rTUmFIAWtmSoZIaIktOKy3L1CZy-L1_PMGSmkqPgpOs95QwhVvCJv0CmVc0wQcob6azvE1JsujAEyDgN2zyn2McceMFXYDB5vJV8W3TR8x84kF4bYG-yg6zL2MIIbwWO7x203xQTZweBgAXIYJ_y8tyn48NuMIQ5v0UlrugyXx36Bvn76-OXmc7F6vL27uV4VTshKFtK0FaHWUvCK8Za3LWtYY2xDjLBESdUAs6YGYR0xTlAPgrTSe1k3vpLW8gv0_nB3m-LPCfKo-5CXD5sB4pS1og1Tqm7mYH0IuhRzTtDqbQq9SXtNiV5U641ejOrFqF5U67-q9W5G3x3fmGwP_h94dDsHPhwCv0IH-_8-rNd3D8s088WBD3mE3Qtv0g9dKa6k_vZwq-_XbCXv109a8j_DwJ1l</recordid><startdate>200406</startdate><enddate>200406</enddate><creator>Kawai, Toshiaki</creator><creator>Hiroi, Sadayuki</creator><creator>Nakanishi, Kuniaki</creator><creator>Sakurai, Yutaka</creator><creator>Torikata, Chikao</creator><general>Blackwell Science Pty</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200406</creationdate><title>Abnormalities in chromosome 17 and p53 in lung carcinoma cells detected by fluorescence in situ hybridization</title><author>Kawai, Toshiaki ; Hiroi, Sadayuki ; Nakanishi, Kuniaki ; Sakurai, Yutaka ; Torikata, Chikao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4565-5af601bb1ed723f3ff2929ab90a4b07579e2ba8e4bc0ac41de40f5dd589d65bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Carcinoma - genetics</topic><topic>Carcinoma - pathology</topic><topic>Carcinoma - surgery</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - pathology</topic><topic>chromosome</topic><topic>Chromosome Aberrations</topic><topic>Chromosomes, Human, Pair 17</topic><topic>Female</topic><topic>fluorescence in situ hybridization</topic><topic>Genes, p53</topic><topic>Humans</topic><topic>immunohistochemistry</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Interphase</topic><topic>lung carcinoma</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - pathology</topic><topic>Lung Neoplasms - surgery</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Staging</topic><topic>p53</topic><topic>Prognosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawai, Toshiaki</creatorcontrib><creatorcontrib>Hiroi, Sadayuki</creatorcontrib><creatorcontrib>Nakanishi, Kuniaki</creatorcontrib><creatorcontrib>Sakurai, Yutaka</creatorcontrib><creatorcontrib>Torikata, Chikao</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawai, Toshiaki</au><au>Hiroi, Sadayuki</au><au>Nakanishi, Kuniaki</au><au>Sakurai, Yutaka</au><au>Torikata, Chikao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abnormalities in chromosome 17 and p53 in lung carcinoma cells detected by fluorescence in situ hybridization</atitle><jtitle>Pathology international</jtitle><addtitle>Pathol Int</addtitle><date>2004-06</date><risdate>2004</risdate><volume>54</volume><issue>6</issue><spage>413</spage><epage>419</epage><pages>413-419</pages><issn>1320-5463</issn><eissn>1440-1827</eissn><abstract>The value of fluorescence in situ hybridization (FISH) as an aid to deciding the prognosis for lung carcinoma patients, comparing quantitatively the signal from the p53 gene (17p13.1) on chromosome 17, was studied. A dual‐labeling technique was used, using probes for the centromeric region of chromosome 17 and for the p53 gene locus. FISH was used on frozen sections of 68 surgically resected lung carcinoma (20 adenocarcinoma; 37 squamous cell carcinoma; 11 large, small, and other cell carcinoma). Hybridization signals were counted for 100–200 interphase nuclei per specimen using a Zeiss confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany) equipped with a bandpass filter for diaminophenolindole and a longpass filter for rhodamine. Clinicopathologic data were evaluated using the Statistical Analysis System. Chromosome 17 polysomy (three or more signals) was greater in poorly differentiated than in well‐differentiated lung carcinoma (P < 0.05). p53 deletion correlated with p53 immunostaining (P < 0.05). Thus, analysis by FISH using DNA probes for chromosome 17 and p53 may be of some, albeit limited, value in determination of prognosis.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Science Pty</pub><pmid>15144400</pmid><doi>10.1111/j.1440-1827.2004.01635.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Pathology international, 2004-06, Vol.54 (6), p.413-419 |
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| language | eng |
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| subjects | Carcinoma - genetics Carcinoma - pathology Carcinoma - surgery Cell Nucleus - genetics Cell Nucleus - pathology chromosome Chromosome Aberrations Chromosomes, Human, Pair 17 Female fluorescence in situ hybridization Genes, p53 Humans immunohistochemistry In Situ Hybridization, Fluorescence - methods Interphase lung carcinoma Lung Neoplasms - genetics Lung Neoplasms - pathology Lung Neoplasms - surgery Male Middle Aged Neoplasm Staging p53 Prognosis |
| title | Abnormalities in chromosome 17 and p53 in lung carcinoma cells detected by fluorescence in situ hybridization |
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