Structure of the O-polysaccharide and serological cross-reactivity of the Providencia stuartii O33 lipopolysaccharide containing 4-( N-acetyl- d-aspart-4-yl)amino-4,6-dideoxy- d-glucose

• Published in: FEMS immunology and medical microbiology 2004-06, Vol.41 (2), p.133-139
• Main Authors: Torzewska, Agnieszka, Kocharova, Nina A, Zatonsky, George V, Blaszczyk, Aleksandra, Bystrova, Olga V, Shashkov, Alexander S, Knirel, Yuriy A, Rozalski, Antoni
• Format: Article
• Language: English
• Subjects: Amino Acids - analysis; Animals; Antibodies, Bacterial - immunology; Bacterial polysaccharide structure; Biodegradation; Carbohydrate Sequence; Carbohydrates - analysis; Cross Reactions; Cross-reactivity; Epitopes; Glucose; Immunoenzyme Techniques; Lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - chemistry; Lipopolysaccharides - immunology; Lipopolysaccharides - isolation & purification; Magnetic resonance spectroscopy; Methylation; Molecular Sequence Data; NMR; NMR spectroscopy; Nuclear magnetic resonance; Nuclear Magnetic Resonance, Biomolecular; O Antigens - chemistry; O Antigens - immunology; O-antigen; Polysaccharides; Protons; Providencia - chemistry; Providencia - immunology; Providencia stuartii; Rabbits; Serological relationship
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1016/j.femsim.2004.02.007

The O-polysaccharide of Providencia stuartii O33 was obtained by mild acid degradation of the lipopolysaccharide and the following structure of the tetrasaccharide repeating unit was established:→ 6)-α- d-Glc pNAc-(1 → 4)-α- d-Gal pA-(1 → 3)-α- d-Glc pNAc-(1 → 3)-β- d-Qui p4N(Ac- d-Asp)-(1 →, where d-Qui4N(Ac- d-Asp) is 4-( N-acetyl- d-aspart-4-yl)amino-4,6-dideoxy- d-glucose. Structural studies were performed using sugar and methylation analyses and NMR spectroscopy, including conventional 2D 1H, 1H COSY, TOCSY, NOESY and 1H, 13C HSQC experiments as well as COSY and NOESY experiments in an H 2O–D 2O mixture to reveal correlations for NH protons. The O-polysaccharide of P. stuartii O33 shares an α- d-Glc p NAc-(1 → 3)-β- d-Qui p4N(Ac- d-Asp) epitope with that of Proteus mirabilis O38, which seems to be responsible for a marked serological cross-reactivity of anti- P. stuartii O33 serum with the lipopolysaccharide of the latter bacterium. P. stuartii O33 is serologically related also to P. stuartii O4, whose O-polysaccharide contains a lateral β- d-Qui4N(Ac- l-Asp) residue.