Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation

• Published in: FEBS letters 2004-05, Vol.566 (1), p.121-125
• Main Authors: Wurm, Susanne, Paar, Christian, Sonnleitner, Alois, Sonnleitner, Max, Höglinger, Otmar, Romanin, Christoph, Wechselberger, Christian
• Format: Article
• Language: English
• Subjects: Actins - metabolism; beta-Cyclodextrins; Calcium - chemistry; Calcium - metabolism; CD3 Complex - chemistry; CD3 Complex - metabolism; Cell Membrane - metabolism; Cholesterol - deficiency; Cholesterol - metabolism; CT-B, cholera toxin subunit B; Cyclodextrins - pharmacology; Cytochalasin D - pharmacology; Enzyme Activation; Fluorescence microscopy; Fluorescent Antibody Technique; G(M1) Ganglioside - chemistry; G(M1) Ganglioside - metabolism; GPI, glycosylphosphatidylinositol; Humans; Hypothermal stimulation; Hypothermia, Induced; Jurkat Cells; Lipid raft; Lymphocyte; Lymphocyte Activation; MAPK, mitogen-activated protein kinase; Mitogen-Activated Protein Kinases - metabolism; MβCD, methyl-β-cyclodextrin; Prion protein; Prions - metabolism; Protein Structure, Tertiary; PrP, prion protein; SAPK, stress-activated protein kinase; T-cell receptor; T-Lymphocytes - metabolism; TCR, T-cell receptor
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2004.03.114

While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 °C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca 2+ mobilization and mitogen-activated protein kinase-phosphorylation.