Spectroscopic investigation on the energy transfer process in photosynthetic apparatus of cyanobacteria

In this work, we employ cyanobacteria, Spirulina platensis, and separate their photosynthetic apparatus, phycobilisome (PBS), thylakoid membrane and phycobilisome–thylakoid membrane complex. The steady state absorption spectra, fluorescence spectra and corresponding deconvoluted spectra and picoseco...

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Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2004-06, Vol.60 (7), p.1543-1547
Main Authors: Li, Ye, Wang, Bei, Ai, Xi-Cheng, Zhang, Xing-Kang, Zhao, Jing-Quan, Jiang, Li-Jin
Format: Article
Language:English
Subjects:
Chlorophyll - chemistry
Chlorophyll - metabolism
Cyanobacteria
Cyanobacteria - metabolism
Energy Transfer
Photosystem I Protein Complex - chemistry
Photosystem I Protein Complex - metabolism
Photosystem II
Photosystem II Protein Complex - chemistry
Photosystem II Protein Complex - metabolism
Phycobilisome
Phycobilisomes - chemistry
Phycobilisomes - metabolism
Spectrometry, Fluorescence
Spectrophotometry
Thylakoids - chemistry
Thylakoids - metabolism
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Summary:In this work, we employ cyanobacteria, Spirulina platensis, and separate their photosynthetic apparatus, phycobilisome (PBS), thylakoid membrane and phycobilisome–thylakoid membrane complex. The steady state absorption spectra, fluorescence spectra and corresponding deconvoluted spectra and picosecond time-resolved spectra are used to investigate the energy transfer process in phycobilisome–thylakoid membrane complex. The results on steady state spectra show chlorophylls of the photosystem II are able to transfer excitation energy to phycobilisome with Chl a molecules selectively excited. The decomposition of the steady state spectra further suggest the uphill energy transfer originate from chlorophylls of photosystem II to cores of phycobilisome, while rods and cores of phycobilisome cannot receive energy from the chlorophylls of photosystem I. The time constant for the back energy transfer process is 18 ps.
ISSN:1386-1425
DOI:10.1016/j.saa.2003.08.017