Cloning and developmental expression of Baf57 in Xenopus laevis

Mammalian and Drosophila homologues of Baf57 have been previously isolated as being a subunit of SWI/SNF-like chromatin remodeling complexes. Here, we report the cloning and developmental expression of Xenopus Baf57. We isolated XBaf57 by using an expression cloning approach to identify novel modula...

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Bibliographic Details
Published in:Mechanisms of development 2002-08, Vol.116 (1), p.177-181
Main Authors: Domingos, Pedro M, Obukhanych, Tetyana V, Altmann, Curtis R, Hemmati-Brivanlou, A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Baf57
Base Sequence
Bone morphogenetic proteins
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - physiology
Cloning, Molecular
DNA, Complementary - genetics
DNA-Binding Proteins - physiology
Embryonic Induction - genetics
Gene Expression Regulation, Developmental
In Situ Hybridization
Molecular Sequence Data
Nervous System - embryology
Neural induction
Sequence Homology, Amino Acid
Smad7
Smad7 Protein
SWI/SNF
Trans-Activators - physiology
Xenopus
Xenopus laevis - embryology
Xenopus laevis - genetics
Xenopus laevis - physiology
Xenopus Proteins
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Summary:Mammalian and Drosophila homologues of Baf57 have been previously isolated as being a subunit of SWI/SNF-like chromatin remodeling complexes. Here, we report the cloning and developmental expression of Xenopus Baf57. We isolated XBaf57 by using an expression cloning approach to identify novel modulators of Xenopus Smad7. XBaf57 co-operates with XSmad7 by increasing the expression of neural markers in ectodermal explants. XBaf57 is expressed in the ectoderm and pre-involuting mesoderm during gastrula stages and in the central nervous system during neurula and tailbud stages. These results raise the possibility that XBaf57 (or XBaf57-containing chromatin remodelling complexes) may be involved in the process of neural induction during Xenopus embryonic development.
ISSN:0925-4773
1872-6356
DOI:10.1016/S0925-4773(02)00129-6