Identification of genes expressed in response to phytoplasma infection in leaves of Prunus armeniaca by messenger RNA differential display

The messenger RNA (mRNA) differential display technique was applied to the identification and isolation of genes whose transcription was altered in leaves of Prunus armeniaca infected by European stone fruit yellows (ESFY) phytoplasma belonging to ribosomal subgroup 16SrX-B. Four genes whose steady-...

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Bibliographic Details
Published in:Gene 2004-05, Vol.332, p.29-34
Main Authors: Carginale, Vincenzo, Maria, Giovanna, Capasso, Clemente, Ionata, Elena, La Cara, Francesco, Pastore, Maria, Bertaccini, Assunta, Capasso, Antonio
Format: Article
Language:English
Subjects:
Amino acid transport
Arabidopsis thaliana
Blotting, Northern
DNA, Complementary - chemistry
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
Gene Expression Profiling
Gene Expression Regulation, Plant
HSP70
Metallothionein
Phytoplasma
Phytoplasma - growth & development
Plant Leaves - genetics
Plant Leaves - microbiology
Prunus - genetics
Prunus - microbiology
Prunus armeniaca
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Analysis, DNA
Stress response
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Summary:The messenger RNA (mRNA) differential display technique was applied to the identification and isolation of genes whose transcription was altered in leaves of Prunus armeniaca infected by European stone fruit yellows (ESFY) phytoplasma belonging to ribosomal subgroup 16SrX-B. Four genes whose steady-state levels of expression significantly changed in response to phytoplasma infection were isolated and identified. The results obtained show that two group of genes are affected by phytoplasma infection in apricot leaves. The first group comprises genes that are up-regulated by phytoplasma presence: in particular, a gene encoding the heat-shock protein HSP-70, a gene encoding a metallothionein (MT) and another homologous to the EST 673 cDNA clone of P. armeniaca, whose function was unknown. The other gene identified in our analysis is down-regulated by phytoplasma presence. It encodes a protein having homology to an amino acid transporter of Arabidopsis thaliana. Our findings demonstrate the usefulness of mRNA differential display approach for the detection of plant metabolic pathways affected by phytoplasma infection.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2004.02.030