Mechanisms of desflurane-induced preconditioning in isolated human right atria In vitro

• Published in: Anesthesiology (Philadelphia) 2002-07, Vol.97 (1), p.33-41
• Main Authors: HANOUZ, Jean-Luc, YVON, Alexandra, MASSETTI, Massimo, LEPAGE, Olivier, BABATASI, Gérard, KHAYAT, André, BRICARD, Henri, GERARD, Jean-Louis
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - pharmacology; Anesthetics, Inhalation - pharmacology; Anesthetics. Neuromuscular blocking agents; Biological and medical sciences; Desflurane; Glyburide - pharmacology; Heart - drug effects; Humans; In Vitro Techniques; Ischemic Preconditioning, Myocardial; Isoflurane - analogs & derivatives; Isoflurane - pharmacology; Medical sciences; Myocardial Contraction - drug effects; Neuropharmacology; Pharmacology. Drug treatments; Potassium Channels - drug effects; Receptors, Adrenergic, alpha - drug effects; Receptors, Adrenergic, beta - drug effects; Receptors, Purinergic P1 - drug effects
• ISSN: 0003-3022; 1528-1175
• DOI: 10.1097/00000542-200207000-00006

The authors examined the role of adenosine triphosphate-sensitive potassium (K(ATP)) channels, adenosine A1 receptor, and alpha and beta adrenoceptors in desflurane-induced preconditioning in human myocardium, in vitro. The authors recorded isometric contraction of human right atrial trabeculae suspended in oxygenated Tyrode's solution (34 degrees C; stimulation frequency, 1 Hz). Before a 30-min anoxic period, 3, 6, and 9% desflurane was administered during 15 min. Desflurane, 6%, was also administered in the presence of 10 microm glibenclamide, a K(ATP) channels antagonist; 10 microm HMR 1098, a sarcolemmal K(ATP) channel antagonist; 800 microm 5-hydroxy-decanoate (5-HD), a mitochondrial K(ATP) channel antagonist; 1 microm phentolamine, an alpha-adrenoceptor antagonist; 1 microm propranolol, a beta-adrenoceptor antagonist; and 100 nm 8-cyclopentyl-1,3-dipropylxanthine (DPX), the adenosine A1 receptor antagonist. Developed force at the end of a 60-min reoxygenation period was compared (mean +/- SD). Desflurane at 3% (95 +/- 13% of baseline), 6% (86 +/- 6% of baseline), and 9% (82 +/- 6% of baseline) enhanced the recovery of force after 60 min of reoxygenation as compared with the control group (50 +/- 11% of baseline). Glibenclamide (60 +/- 12% of baseline), 5-HD (57 +/- 21% of baseline), DPX (63 +/- 19% of baseline), phentolamine (56 +/- 20% of baseline), and propranolol (63 +/- 13% of baseline) abolished desflurane-induced preconditioning. In contrast, HMR 1098 (85 +/- 12% of baseline) did not modify desflurane-induced preconditioning. In vitro, desflurane preconditions human myocardium against simulated ischemia through activation of mitochondrial K(ATP) channels, adenosine A1 receptor, and alpha and beta adrenoceptors.