An analysis of the performance characteristics of serological tests for the diagnosis of Neospora caninum infection in cattle

Aims: To analyse the performance characteristics (sensitivity/specificity) of two enzyme-linked immunosorbent assays (ELISA) against the indirect fluorescent antibody test (IFAT). Methods: A total of 1199 sera were tested in two ELISAs and the IFAT and results analysed utilising software that perfor...

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Bibliographic Details
Published in:Veterinary parasitology 2002-08, Vol.107 (3), p.197-207
Main Authors: Reichel, M.P, Pfeiffer, D.U
Format: Article
Language:English
Subjects:
Animals
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - epidemiology
Cattle Diseases - parasitology
Coccidiosis - diagnosis
Coccidiosis - epidemiology
Coccidiosis - parasitology
Coccidiosis - veterinary
ELISA
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Fluorescent Antibody Technique, Indirect - veterinary
IFAT
Neospora - isolation & purification
Neospora caninum
New Zealand - epidemiology
Protozoa
ROC Curve
ROC-analysis
Sensitivity and Specificity
Seroepidemiologic Studies
Serology
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Summary:Aims: To analyse the performance characteristics (sensitivity/specificity) of two enzyme-linked immunosorbent assays (ELISA) against the indirect fluorescent antibody test (IFAT). Methods: A total of 1199 sera were tested in two ELISAs and the IFAT and results analysed utilising software that performed a receiver-operating characteristic (ROC) analysis. Results: Sensitivity and specificity for the two ELISAs were calculated for a range of different cut-offs. Minimal misclassification was achieved at cut-offs that, in the case of the Central Animal Health Laboratory (CAHL)-ELISA were in line with previously published cut-off values. In the case of the IDEXX-ELISA lower cut-off values than suggested by the manufacturer were calculated. Conclusions: ROC-analysis resulted in optimised, as compared to the IFAT, cut-off thresholds for the CAHL and IDEXX-ELISA which can be further adjusted depending on the purpose of the investigation.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(02)00086-9