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Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis

Ribosomal proteins (RP) S13 and RPL23 were previously identified as two upregulated genes in a multidrug-resistant gastric cancer cell line SGC7901/VCR compared to its parental cell SGC7901 by differential display PCR. The aim of this study was to explore the roles of RPS13 and RPL23 in multidrug re...

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Published in:Experimental cell research 2004-06, Vol.296 (2), p.337-346
Main Authors: Shi, Yongquan, Zhai, Huihong, Wang, Xin, Han, Zheyi, Liu, Changjiang, Lan, Mei, Du, Jingping, Guo, Changcun, Zhang, Yumei, Wu, Kaichun, Fan, Daiming
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cited_by cdi_FETCH-LOGICAL-c452t-e41d8edb491ae3f322fda3bd17cbe0e2da78b5f6952a96216441968ae1a64c223
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container_title Experimental cell research
container_volume 296
creator Shi, Yongquan
Zhai, Huihong
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Guo, Changcun
Zhang, Yumei
Wu, Kaichun
Fan, Daiming
description Ribosomal proteins (RP) S13 and RPL23 were previously identified as two upregulated genes in a multidrug-resistant gastric cancer cell line SGC7901/VCR compared to its parental cell SGC7901 by differential display PCR. The aim of this study was to explore the roles of RPS13 and RPL23 in multidrug resistance (MDR) in gastric cancer cells. RPS13 and RPL23 were genetically overexpressed in SGC7901 cells, respectively. Either RPS13 or RPL23 enhanced resistance of SGC7901 cells to vincristine, adriamycin, and 5-fludrouracil. RPL23 also enhanced resistance of SGC7901 cells to cisplatin. Overexpression of either RPS13 or RPL23 did not alter the population doubling time, [ 3H]leucine incorporation, and intracellular adriamycin accumulation of SGC7901 cells. However, either RPS13 or RPL23 could protect SGC7901 cells from undergoing vincristine-induced apoptosis. Western blot analysis revealed that both RPS13 and RPL23 significantly increased the expression level of Bcl-2 and Bcl-2/Bax ratio in SGC7901 cells. In addition, overexpression of RPL23 enhanced glutathione S-transferase (GST) activity and intracellular glutathione content in SGC7901 cells. Together, this work demonstrates that either RPS13 or RPL23 can promote MDR in gastric cancer cells by suppressing drug-induced apoptosis, and that RPL23 may also promote MDR in gastric cancer cells through regulation of glutathione S-transferase-mediated drug-detoxifying system.
doi_str_mv 10.1016/j.yexcr.2004.02.009
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subjects Apoptosis
Apoptosis - drug effects
bcl-2-Associated X Protein
Cell Division
Cell Line, Tumor
Drug Resistance, Multiple
Gastric cancer
Glutathione Transferase - metabolism
Humans
Multidrug resistance
Neoplasm Proteins - physiology
Proto-Oncogene Proteins - analysis
Proto-Oncogene Proteins c-bcl-2 - analysis
Ribosomal protein L23
Ribosomal protein S13
Ribosomal Proteins - genetics
Ribosomal Proteins - physiology
Stomach Neoplasms - pathology
Transfection
Vincristine - pharmacology
title Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis
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